Product Info Summary
SKU: | M00401-1 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352
SKU/Catalog Number
M00401-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352 (Catalog # M00401-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00401-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM352
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human MUM1/IRF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human MUM1/IRF4 (Interferon regulatory factor 4).
Reactive Species
M00401-1 is reactive to IRF4 in Human
Applications
M00401-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
51.772kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:800 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of Raji cell lysate using anti-MUM1/IRF4 rabbit monoclonal antibody (Clone RM352) at a 1:1000 dilution.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-MUM1/IRF4 rabbit monoclonal antibody (Clone RM352) at a 1:250 dilution.
Click image to see more details
Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human melanoma tissue section using anti-MUM1/IRF4 rabbit monoclonal antibody (Clone RM352) at a 1:250 dilution.
Protein Target Info & Infographic
Gene/Protein Information For IRF4 (Source: Uniprot.org, NCBI)
Gene Name
IRF4
Full Name
Interferon regulatory factor 4
Weight
51.772kDa
Superfamily
IRF family
Alternative Names
interferon regulatory factor 4; IRF4; IRF-4; LSIRF; LSIRFMUM1; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5 IRF4 LSIRF, MUM1, NF-EM5, SHEP8 interferon regulatory factor 4 interferon regulatory factor 4|lymphocyte-specific interferon regulatory factor|multiple myeloma oncogene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF4, check out the IRF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352 (M00401-1)
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No publications found for M00401-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352
Question
My question regards using your anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 for positive regulation of interleukin-2 biosynthetic process studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We appreciate your inquiry. This M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 is validated on raji cell lysate, human melanoma tissue, thyroid cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-27
Question
We have observed staining in human lymph node. What should we do? Is anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 supposed to stain lymph node positively?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
From what I have seen in literature lymph node does express IRF4. From what I have seen in Uniprot.org, IRF4 is expressed in endocervix, spleen, skin, lymph node, among other tissues. Regarding which tissues have IRF4 expression, here are a few articles citing expression in various tissues:
Lymph node, Pubmed ID: 12651066
Skin, Pubmed ID: 15489334
Spleen, Pubmed ID: 8921401, 9326949
Boster Scientific Support
Answered: 2020-04-13
Question
I was wanting to use your anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 for WB for human lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
You can see on the product datasheet, M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-13
Question
I was wanting to use to test anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 on human lymph node for research purposes, then I may be interested in using anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The products we sell, including anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-24
Question
We ordered your anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
I looked at the website and datasheets of our anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-10-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
Will anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 work for WB with lymph node?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
According to the expression profile of lymph node, IRF4 is highly expressed in lymph node. So, it is likely that anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 will work for WB with lymph node.
Boster Scientific Support
Answered: 2019-01-14
Question
Is a blocking peptide available for product anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 (M00401-1)?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
We do provide the blocking peptide for product anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 (M00401-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-10
Question
See below the WB image, lot number and protocol we used for lymph node using anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1. Please let me know if you require anything else.
B. Rodriguez
Verified customer
Asked: 2018-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-11
Question
Can you help my question with product M00401-1, anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-30
Question
I see that the anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-22
Question
Do you have a BSA free version of anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 available?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-24
Question
Our lab were happy with the WB result of your anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone
A. Huang
Verified customer
Asked: 2017-07-26
Answer
From what I have seen in literature, lymph node does express IRF4. Generally IRF4 expresses in nucleus. Regarding which tissues have IRF4 expression, here are a few articles citing expression in various tissues:
Lymph node, Pubmed ID: 12651066
Skin, Pubmed ID: 15489334
Spleen, Pubmed ID: 8921401, 9326949
Boster Scientific Support
Answered: 2017-07-26
Question
Would M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Wu
Verified customer
Asked: 2015-07-01
Answer
As indicated on the product datasheet, M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-01
Question
Is this M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 reactive to the isotypes of IRF4?
S. Walker
Verified customer
Asked: 2014-02-20
Answer
The immunogen of M00401-1 anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 is A peptide corresponding to the C-terminus of human MUM1/IRF4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-20
Question
We are currently using anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 M00401-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
S. Jackson
Verified customer
Asked: 2013-02-05
Answer
The anti-MUM1/IRF4 Rabbit Monoclonal antibody, Clone#RM352 (M00401-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-05