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Product Info Summary
| SKU: | M01697 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody
View all Myosin light chain kinase Antibodies
SKU/Catalog Number
M01697
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody catalog # M01697. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01697)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
G-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myosin light chain kinase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01697 is reactive to MYLK in Human, Mouse, Rat
Applications
M01697 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
v
Calculated molecular weight
210.715kDa
Background of Myosin light chain kinase
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of myosin light chain kinase expression in HUVEC cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MYLK (Source: Uniprot.org, NCBI)
Gene Name
MYLK
Full Name
Myosin light chain kinase, smooth muscle
Weight
210.715kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp686I10125; EC 2.7.11; EC 2.7.11.18; FLJ12216; Kinase-related protein; KRP; light polypeptide kinase; MLCK1; MLCK108; MLCKMLCK210; MSTP083; MYLK1; myosin light chain kinase; smooth muscle myosin light chain kinase; smooth muscle; telokin MYLK AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MMIHS, MSTP0831, smMLCK, MYLK myosin light chain kinase myosin light chain kinase, smooth muscle|kinase-related protein|myosin, light polypeptide kinase|smooth muscle myosin light chain kinase|telokin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYLK, check out the MYLK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYLK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody (M01697)
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No publications found for M01697
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Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Myosin light chain kinase MYLK Rabbit Monoclonal Antibody
Question
Does anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 work on dog WB with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Our lab technicians have not tested anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog umbilical vein endothelial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-18
Question
See below the WB image, lot number and protocol we used for umbilical vein using anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein using anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
I would like to test anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 on human umbilical vein for research purposes, then I may be interested in using anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The products we sell, including anti-Myosin light chain kinase Rabbit Monoclonal antibody M01697, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-12
