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Product Info Summary
| SKU: | M02890 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-NAA10 Antibody (Center)
SKU/Catalog Number
M02890
Size
50 µl
Description
Boster Bio Anti-NAA10 Antibody (Center) (Catalog # M02890). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NAA10 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02890)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NAA10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 135-167 amino acids from the Central region of human NAA10.
Reactive Species
M02890 is reactive to NAA10 in Human
Calculated molecular weight
26.5 kDa
Background of NAA10
Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02890 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling NAA10 with M02890 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-NAA10 Antibody (Center) at 1:2000 dilution
Lane 1: SK-BR-3 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HCT116 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-NAA10 Antibody (Center) (M02890)
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