Anti-NADH2/Mtnd2 Antibody Picoband™

Product Name

Anti-NADH2/Mtnd2 Antibody Picoband™

View all Mtnd2 Antibodies

SKU/Catalog Number

A32839

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NADH2/Mtnd2 Antibody Picoband™ catalog # A32839. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NADH2/Mtnd2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32839)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse NADH2/Mtnd2, which shares 80% amino acid (aa) sequence identity with rat NADH2/Mtnd2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A32839 is reactive to Mtnd2 in Mouse, Rat

Applications

A32839 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

38.752kDa

Background of Mtnd2

Mitochondrially encoded NADH dehydrogenase 2 is protein that in humans is encoded by the mitochondrial gene MT-ND2 gene. The ND2 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of MT-ND2 are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Mtnd2 using anti-Mtnd2 antibody (A32839).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mtnd2 antigen affinity purified polyclonal antibody (Catalog # A32839) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mtnd2 at approximately 39KD. The expected band size for Mtnd2 is at 39KD.

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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Mtnd2 antibody (A32839).
Overlay histogram showing ANA-1 cells stained with A32839 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mtnd2 Antibody (A32839, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of NRK cells using anti-Mtnd2 antibody (A32839).
Overlay histogram showing NRK cells stained with A32839 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mtnd2 Antibody (A32839, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Mtnd2 (Source: Uniprot.org, NCBI)

Alternative Names

NADH-ubiquinone oxidoreductase chain 2

For more info on Mtnd2, check out the Mtnd2 Infographic

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In this infographic, you will see the following information for Mtnd2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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