Anti-NALP1 NLRP1 Antibody

Product Name

Anti-NALP1 NLRP1 Antibody

View all NLRP1/NALP1 Antibodies

SKU/Catalog Number

A00777

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NALP1 NLRP1 Antibody (Catalog # A00777). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

NALP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NALP1 NLRP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00777)

Host

Rabbit

Contents

NALP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1. The immunogen is located within the last 50 amino acids of NALP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SOX10 Antibody is predicted to not cross-react with other SOX familiy members.

Reactive Species

A00777 is reactive to NLRP1 in Human

Applications

A00777 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

165.866kDa

Background of NLRP1/NALP1

NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1

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Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 μg/mL.

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Immunofluorescence of NALP1 in K562 cells with NALP1 antibody at 20 μg/mL.

Gene/Protein Information For NLRP1 (Source: Uniprot.org, NCBI)

Alternative Names

CARD7; CARD7SLEV1; Caspase recruitment domain-containing protein 7; CLR17.1; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAPVAMAS1; DKFZp586O1822; KIAA0926DEFCAP-L/S; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1; NACPP1044; NALP1; NALP1caspase recruitment domain protein 7; NLR family, pyrin domain containing 1; NLRP1; Nucleotide-binding domain and caspase recruitment domain; nucleotide-binding oligomerization d NLRP1 AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1 NLR family pyrin domain containing 1 NACHT, LRR and PYD domains-containing protein 1|NACHT, LRR and PYD containing protein 1|NACHT, leucine rich repeat and PYD (pyrin domain) containing 1|NACHT, leucine rich repeat and PYD containing 1|caspase recruitment domain protein 7|caspase recruitment domain-containing protein 7|death effector filament-forming Ced-4-like apoptosis protein|nucleotide-binding domain and caspase recruitment domain|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1

For more info on NLRP1, check out the NLRP1 Infographic

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In this infographic, you will see the following information for NLRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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