Anti-NALP4 NLRP4 Antibody

Product Name

Anti-NALP4 NLRP4 Antibody

View all NALP4 Antibodies

SKU/Catalog Number

A08477

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NALP4 NLRP4 Antibody (Catalog # A08477). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

NALP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NALP4 NLRP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08477)

Host

Rabbit

Contents

NALP4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

NALP4 antibody was raised against a 13 amino acid synthetic peptide near the center of human NALP4. The immunogen is located within amino acids 330 - 380 of NALP4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

GLIPR1 antibody is human and mouse reactive. At least two isoforms of GLIPR1 are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other GLIPR or GLIPR-like proteins.

Reactive Species

A08477 is reactive to NLRP4 in Human, Mouse

Applications

A08477 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

113.415kDa

Background of NALP4

NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP4, also known as PYRIN-containing APAF1-like protein 4, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. In transfected 293 cells, NALP4 suppressed NF-κB induction by TNF-α and IL-1β, suggesting NALP4 operates at a point of convergence in these two signaling pathways. NALP4 also suppressed NF-κB induction resulting from overexpression of several adapter proteins and protein kinases involved in these pathways, including TRAF2, TRAF6, RIP, and IRAK2, as well as the IKK-alpha and IKK-beta, suggesting that NALP4 is critical in modulating NF-κB activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NALP4 antibody can be used for detection of NALP4 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of NALP4 in K562 cell lysate with NALP4 antibody at 1 μg/mL.

Gene/Protein Information For NLRP4 (Source: Uniprot.org, NCBI)

Alternative Names

Cancer/testis antigen 58; CLR19.5; CT58PYRIN and NACHT-containing protein 2; FLJ32126; NACHT, leucine rich repeat and PYD containing 4; NACHT, LRR and PYD domains-containing protein 4; NALP4NACHT, LRR and PYD containing protein 4; NLR family, pyrin domain containing 4; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 4; PAN2Ribonuclease inhibitor 2; PYPAF4PAAD and NACHT-containing protein 2; RNH2PYRIN-containing APAF1-like protein 4 NLRP4 CLR19.5, CT58, NALP4, PAN2, PYPAF4, RNH2 NLR family pyrin domain containing 4 NACHT, LRR and PYD domains-containing protein 4|NACHT, leucine rich repeat and PYD containing 4|PAAD and NACHT-containing protein 2|PYRIN and NACHT-containing protein 2|PYRIN-containing APAF1-like protein 4|cancer/testis antigen 58|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 4|ribonuclease inhibitor 2

For more info on NLRP4, check out the NLRP4 Infographic

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In this infographic, you will see the following information for NLRP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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