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Product Info Summary
| SKU: | A05618-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-NDUFS2 Antibody (Center)
SKU/Catalog Number
A05618-1
Size
80 µl
Description
Boster Bio Anti-NDUFS2 Antibody (Center) (Catalog # A05618-1). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NDUFS2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05618-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NDUFS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human NDUFS2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05618-1 is reactive to NDUFS2 in Human, Mouse
Reconstitution
Calculated molecular weight
52546 Da
Background of NDUFS2
NDUFS2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05618-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of lysates from A431, Hela cell line, mouse brain and rat brain tissue lysate (from left to right), using NDUFS2 Antibody (Center). A05618-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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NDUFS2 Antibody (Center) (Cat. #A05618-1) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For NDUFS2 (Source: Uniprot.org, NCBI)
Gene Name
NDUFS2
Full Name
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
Weight
52546 Da
Superfamily
complex I 49 kDa subunit family
Alternative Names
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, Complex I-49kD, CI-49kD, NADH-ubiquinone oxidoreductase 49 kDa subunit, NDUFS2 NDUFS2 CI-49, MC1DN6 NADH:ubiquinone oxidoreductase core subunit S2 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial|CI-49kD|NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)|NADH-ubiquinone oxidoreductase 49 kDa subunit|NADH-ubiquinone oxidoreductase NDUFS2 subunit|complex 1, mitochondrial respiratory chain, 49-KD subunit|complex I 49kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFS2, check out the NDUFS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFS2 Antibody (Center) (A05618-1)
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