Anti-PVRL4/NECTIN4 Antibody Picoband™

Product Name

Anti-PVRL4/NECTIN4 Antibody Picoband™

View all Nectin-4/PVRL4 Antibodies

SKU/Catalog Number

PB10091

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PVRL4/NECTIN4 Antibody Picoband™ catalog # PB10091. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PVRL4/NECTIN4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10091)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Nectin-4/PVRL4, different from the related mouse sequence by seven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10091 is reactive to Nectin4 in Human

Applications

PB10091 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

66 kDa

Calculated molecular weight

55.657kDa

Background of Nectin-4/PVRL4

PVRL4, also known as Nectin-4, is expressed in human skin, hair follicles, and cultured keratinocytes, but not in fibroblasts. This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Nectin-4/PVRL4 using anti-Nectin-4/PVRL4 antibody (PB10091).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: MM453 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin-4/PVRL4 antigen affinity purified polyclonal antibody (Catalog # PB10091) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin-4/PVRL4 at approximately 66 kDa. The expected band size for Nectin-4/PVRL4 is at 55 kDa.

Gene/Protein Information For Nectin4 (Source: Uniprot.org, NCBI)

Alternative Names

EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4 Nectin4|1200017F15Rik, P, Prr4, Pvrl4|nectin cell adhesion molecule 4|nectin-4|ig superfamily receptor LNIR|nectin 4|poliovirus receptor-related 4|poliovirus receptor-related protein 4

For more info on Nectin4, check out the Nectin4 Infographic

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In this infographic, you will see the following information for Nectin4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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