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- Table of Contents
Nefh antibody (M05307-10) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Designing an IHC experiment for NEFH? The guide covers the expected staining pattern, antigen retrieval, antibodies backed by real IHC images, tissue controls, and protocols taken from published papers.
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Anti-NEFH Antibody (Monoclonal, 35N99)
Nefh antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IHC, WB |
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
1-2μg/ml
Human, mouse, rat
Immunohistochemistry (Frozen Section)
1-2μg/ml
Human, mouse, rat, -
Western blot
0.5ml
Human, mouse, rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-NEFH Antibody (Monoclonal, 35N99)
View all NEFH Antibodies
View all NEFH Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
35N99
Isotype
Mouse IgG1
Immunogen
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Calculated Molecular Weight
115.4 kDa
Background of Nefh
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).
Cite This Product
Anti-NEFH Antibody (Monoclonal, 35N99) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05307-10)
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Product Promise
We promise Anti-NEFH Antibody (Monoclonal, 35N99) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M05307-10
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026