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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-NEFH/Nf H Rabbit Monoclonal Antibody
Boster Bio Anti-NEFH/Nf H Rabbit Monoclonal Antibody catalog # M05307-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEFH/Nf H Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05307-2)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human NEFH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M05307-2 is reactive to NEFH in Human, Mouse, Rat
M05307-2 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of NEFH expression in human brain lysate (M05307-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEFH monoclonal antibody (Catalog # M05307-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEFH
Gene/Protein Information For NEFH (Source: Uniprot.Org, NCBI)
Neurofilament heavy polypeptide
intermediate filament family
200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H NEFH CMT2CC, NFH neurofilament heavy neurofilament heavy polypeptide|200 kDa neurofilament protein|NF-H|neurofilament triplet H protein|neurofilament, heavy polypeptide 200kDa*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NEFH, check out the NEFH Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NEFH: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M05307-2 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
M05307-2 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Neuron-derived IgG Protects Neurons from Complement-dependent Cytotoxicity
An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
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