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Product Info Summary
| SKU: | A11147 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Neudesin NENF Antibody
SKU/Catalog Number
A11147
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Neudesin NENF Antibody (Catalog # A11147). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NENF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Neudesin NENF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11147)
Host
Rabbit
Contents
NENF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF. The immunogen is located within the last 50 amino acids of NENF.
Reactive Species
A11147 is reactive to NENF in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
18.9 kDa
Background of NENF
NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11147 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 μg/mL.
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Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 μg/mL.
Specific Publications For Anti-Neudesin NENF Antibody (A11147)
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