Click image to see more details
Product Info Summary
| SKU: | M02930-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Neuron Specific Enolase (NSE) Eno2 Antibody
View all Enolase 2/Neuron-specific Enolase Antibodies
SKU/Catalog Number
M02930-2
Size
100ul
Description
Boster Bio Anti-Neuron Specific Enolase (NSE) Eno2 Antibody catalog # M02930-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuron Specific Enolase (NSE) Eno2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02930-2)
Host
Rabbit
Contents
Antibody is supplied as an aliquot of serum
Clonality
Polyclonal
Immunogen
Recombinant full length human NSE purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02930-2 is reactive to ENO2 in Human, Mouse, Rat
Applications
M02930-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47.269kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IF/ICC and IHC: 1:500
Validation Images & Assay Conditions
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Western blot analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), M02930-2, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.
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Immunofluorescent analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), RPCA-NSE, dilution 1:500 in red, and costained with chicken pAb to GFAP, CPCA-GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.
Protein Target Info & Infographic
Gene/Protein Information For ENO2 (Source: Uniprot.org, NCBI)
Gene Name
ENO2
Full Name
Gamma-enolase
Weight
47.269kDa
Superfamily
enolase family
Alternative Names
2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific enolase; NSE ENO2 HEL-S-279, NSE enolase 2 gamma-enolase|2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|enolase 2 (gamma, neuronal)|epididymis secretory protein Li 279|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neuronal enriched enolase|neurone-specific enolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENO2, check out the ENO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neuron Specific Enolase (NSE) Eno2 Antibody (M02930-2)
Hello CJ!
M02930-2 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neuroprotective effects of salvianolic acid B against oxygenglucose deprivation/reperfusion damage in primary rat cortical neurons
Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells
Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25%u201335)-induced neuronal injury
%u03B2-Elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells
Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons
Huang H, Jiang L, Li S, Deng J, Li Y, Yao J, Li B, Zheng J. Biomicrofluidics. 2014 Feb 19;8(1):014108. Doi: 10.1063/1.4864235. Ecollection 2014. Using Microfluidic Chip To Form Brain-Derived Neurotrophic Factor Concentration Gradient For Studying ...
Zhang Z, Zhou X, Zhou X, Xu X, Liao M, Yan L, Lv R, Luo H. Neural Regen Res. 2012 May 5;7(13):971-7. Doi: 10.3969/J.Issn.1673-5374.2012.13.002. Methyl 3,4-Dihydroxybenzoate Promotes Neurite Outgrowth Of Cortical Neurons Cultured In Vitro.
Xiao F, Lin Lf, Cheng X, Gao Q, Luo Hm. Mol Med Rep. 2012 Mar;5(3):619-24. Doi: 10.3892/Mmr.2011.692. Epub 2011 Dec 1. Nogo-66 Receptor Activation Inhibits Neurite Outgrowth And Increases ??-Amyloid Protein Secretion Of Cortical Neurons.
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