Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody

Product Name

Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody

View all NGFR/TNFRSF16/p75NTR Antibodies

SKU/Catalog Number

M01187

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody (Catalog # M01187). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Baboon, Cat, Rabbit, Ferret.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01187)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM299

Isotype

IgG1, kappa

Immunogen

NGFR from A875 melanoma cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01187 is reactive to NGFR in Ferret, Human, Monkey, Rabbit, Cat, Baboon

Applications

M01187 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.183kDa

Background of NGFR/TNFRSF16/p75NTR

This monoclonal antibody recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Click image to see more details

Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-NGFR Monoclonal Antibody (SPM299).

Gene/Protein Information For NGFR (Source: Uniprot.org, NCBI)

Alternative Names

CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16 NGFR CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR nerve growth factor receptor tumor necrosis factor receptor superfamily member 16|NGF receptor|TNFR superfamily, member 16|low affinity neurotrophin receptor p75NTR|low-affinity nerve growth factor receptor|p75 ICD

For more info on NGFR, check out the NGFR Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the NGFR Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis