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Product Info Summary
| SKU: | A12284-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Nicalin NCLN Antibody
SKU/Catalog Number
A12284-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Nicalin NCLN Antibody (Catalog # A12284-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Nicalin NCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12284-1)
Host
Rabbit
Contents
NCLN Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN. The immunogen is located within amino acids 390 - 440 of NCLN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ORMDL1 antibody may cross-react with other ORMDL protein family members.
Reactive Species
A12284-1 is reactive to NCLN in Human, Mouse, Rat
Applications
A12284-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
62.974kDa
Background of Nicalin
Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.
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Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NCLN (Source: Uniprot.org, NCBI)
Gene Name
NCLN
Full Name
Nicalin
Weight
62.974kDa
Superfamily
nicastrin family
Alternative Names
Ncl-1; NCLN; NET59; nicalin homolog (zebrafish); nicalin homolog (zebrefish); Nicalin; Nicastrin-like protein NCLN NET59 nicalin nicalin|nicalin homolog|nicastrin-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCLN, check out the NCLN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nicalin NCLN Antibody (A12284-1)
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