Product Info Summary
SKU: | A00428-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Niemann Pick C1/NPC1 Antibody Picoband™
View all Niemann-Pick C1 Antibodies
SKU/Catalog Number
A00428-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Niemann Pick C1/NPC1 Antibody Picoband™ catalog # A00428-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Niemann Pick C1/NPC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00428-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Niemann Pick C1 recombinant protein (Position: A1022-F1278).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00428-2 is reactive to NPC1 in Human, Mouse, Rat
Applications
A00428-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
170 kDa
Calculated molecular weight
142.167kDa
Background of Niemann-Pick C1
Niemann-Pick disease, type C1 (NPC1) is a membrane protein that mediates intracellular cholesterol trafficking in mammals. In humans it is encoded by the NPC1 gene (chromosome location 18q11). This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Direct ELISA,0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody (A00428-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1 antigen affinity purified polyclonal antibody (Catalog # A00428-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C1 at approximately 170KD. The expected band size for Niemann Pick C1 is at 142KD.
Protein Target Info & Infographic
Gene/Protein Information For NPC1 (Source: Uniprot.org, NCBI)
Gene Name
NPC1
Full Name
NPC intracellular cholesterol transporter 1
Weight
142.167kDa
Superfamily
patched family
Alternative Names
FLJ98532; Niemann-Pick C1 protein; Niemann-Pick disease, type C1; Niemann-Pick Type C1; NPC; NPC1; SLC65A1 NPC1 NPC, POGZ, SLC65A1 NPC intracellular cholesterol transporter 1 NPC intracellular cholesterol transporter 1|Niemann-Pick C1 protein|truncated Niemann-Pick C1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPC1, check out the NPC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Niemann Pick C1/NPC1 Antibody Picoband™ (A00428-2)
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No publications found for A00428-2
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Niemann Pick C1/NPC1 Antibody Picoband™
Question
Would anti-Niemann Pick C1/NPC1 antibody A00428-2 work on primate ELISA with liver?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Our lab technicians have not tested anti-Niemann Pick C1/NPC1 antibody A00428-2 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Niemann Pick C1/NPC1 antibody A00428-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-31
Question
We want to test anti-Niemann Pick C1/NPC1 antibody A00428-2 on human secondary oocyte for research purposes, then I may be interested in using anti-Niemann Pick C1/NPC1 antibody A00428-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The products we sell, including anti-Niemann Pick C1/NPC1 antibody A00428-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-12
Question
Will A00428-2 anti-Niemann Pick C1/NPC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, A00428-2 anti-Niemann Pick C1/NPC1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
We bought anti-Niemann Pick C1/NPC1 antibody for WB on secondary oocyte in a previous project. I am using mouse, and I plan to use the antibody for ELISA next. We want examining secondary oocyte as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
I looked at the website and datasheets of our anti-Niemann Pick C1/NPC1 antibody and I see that A00428-2 has been tested on mouse in both WB and ELISA. Thus A00428-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-13
Question
Will anti-Niemann Pick C1/NPC1 antibody A00428-2 work for WB with secondary oocyte?
G. Collins
Verified customer
Asked: 2019-10-31
Answer
According to the expression profile of secondary oocyte, NPC1 is highly expressed in secondary oocyte. So, it is likely that anti-Niemann Pick C1/NPC1 antibody A00428-2 will work for WB with secondary oocyte.
Boster Scientific Support
Answered: 2019-10-31
Question
My question regarding product A00428-2, anti-Niemann Pick C1/NPC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00428-2 anti-Niemann Pick C1/NPC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-08
Question
We have observed staining in rat cerebellum. Do you have any suggestions? Is anti-Niemann Pick C1/NPC1 antibody supposed to stain cerebellum positively?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
According to literature cerebellum does express NPC1. According to Uniprot.org, NPC1 is expressed in secondary oocyte, cerebellum, placenta, liver, among other tissues. Regarding which tissues have NPC1 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 14702039
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 15489334, 17897319
Boster Scientific Support
Answered: 2019-07-09
Question
Is this A00428-2 anti-Niemann Pick C1/NPC1 antibody reactive to the isotypes of NPC1?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The immunogen of A00428-2 anti-Niemann Pick C1/NPC1 antibody is E. coli-derived human Niemann Pick C1 recombinant protein (Position: A1022-F1278). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-03
Question
Please see the WB image, lot number and protocol we used for secondary oocyte using anti-Niemann Pick C1/NPC1 antibody A00428-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-Niemann Pick C1/NPC1 antibody A00428-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
The anti-Niemann Pick C1/NPC1 antibody (A00428-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-14
Question
My colleagues were happy with the WB result of your anti-Niemann Pick C1/NPC1 antibody. However we have observed positive staining in cerebellum late endosome membrane using this antibody. Is that expected? Could you tell me where is NPC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
From literature, cerebellum does express NPC1. Generally NPC1 expresses in late endosome membrane. Regarding which tissues have NPC1 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 14702039
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 15489334, 17897319
Boster Scientific Support
Answered: 2017-08-02
Question
Do you have a BSA free version of anti-Niemann Pick C1/NPC1 antibody A00428-2 available?
O. Brown
Verified customer
Asked: 2017-06-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Niemann Pick C1/NPC1 antibody A00428-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-16
Question
Our lab want to know about using your anti-Niemann Pick C1/NPC1 antibody for intracellular cholesterol transport studies. Has this antibody been tested with western blotting on rat pancreas tissue? We would like to see some validation images before ordering.
A. Jackson
Verified customer
Asked: 2016-04-25
Answer
Thank you for your inquiry. This A00428-2 anti-Niemann Pick C1/NPC1 antibody is validated on rat pancreas tissue, mouse nih3t3 whole cell lysates. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-04-25
Question
Is a blocking peptide available for product anti-Niemann Pick C1/NPC1 antibody (A00428-2)?
K. Krishna
Verified customer
Asked: 2016-02-09
Answer
We do provide the blocking peptide for product anti-Niemann Pick C1/NPC1 antibody (A00428-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-09
Question
I see that the anti-Niemann Pick C1/NPC1 antibody A00428-2 works with WB, what is the protocol used to produce the result images on the product page?
A. Taylor
Verified customer
Asked: 2015-12-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for secondary oocyte using anti-Niemann Pick C1/NPC1 antibody A00428-2. Let me know if you need anything else.
C. Carter
Verified customer
Asked: 2015-07-22
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-22
Question
I was wanting to use your anti-Niemann Pick C1/NPC1 antibody for WB for human secondary oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human secondary oocyte identification?
J. Parker
Verified customer
Asked: 2015-03-10
Answer
It shows on the product datasheet, A00428-2 anti-Niemann Pick C1/NPC1 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human secondary oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-10