Anti-Notchless protein homolog 1 NLE1 Antibody

NLE1 antibody

Boster Bio Anti-Notchless protein homolog 1 NLE1 Antibody (Catalog # A09529). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A09529
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Notchless protein homolog 1 NLE1 Antibody

View all NLE1 Antibodies

SKU/Catalog Number

A09529

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Notchless protein homolog 1 NLE1 Antibody (Catalog # A09529). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Notchless protein homolog 1 NLE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09529)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human NLE1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09529 is reactive to NLE1 in Human, Mouse, Rat

Applications

A09529 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

53.32kDa

Background of NLE1

Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Bienvenut W.V.; Submitted (AUG-2005) to UniProtKB.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For NLE1 (Source: Uniprot.org, NCBI)

Gene Name

NLE1

Full Name

Notchless protein homolog 1

Weight

53.32kDa

Superfamily

NLE1/RSA4 family

Alternative Names

FLJ10458; Nle; Notchless gene homolog; notchless homolog 1 (Drosophila); notchless protein homolog 1 NLE1 NLE notchless homolog 1 notchless protein homolog 1|Notchless gene homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NLE1, check out the NLE1 Infographic

NLE1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NLE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Notchless protein homolog 1 NLE1 Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung placenta using anti-NLE1 antibody A09529. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-17

Question

We are currently using anti-NLE1 antibody A09529 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

The anti-NLE1 antibody (A09529) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-27

Question

Is a blocking peptide available for product anti-NLE1 antibody (A09529)?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

We do provide the blocking peptide for product anti-NLE1 antibody (A09529). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-10

Order DetailsPrice
A09529-100uL

100uL

$360

Free Secondary Antibody

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A09529
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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