Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1096) Antibody

Product Name

Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1096) Antibody

View all NMDAR2C Antibodies

SKU/Catalog Number

P07769

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1096) Antibody (Catalog # P07769). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1096) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07769)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1096 of the NR2C subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P07769 is reactive to GRIN2C in Mouse, Rat

Applications

P07769 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

134.209kDa

Background of NMDAR2C

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser-1096 by PKB on NR2C has been recently demonstrated to regulate NMDA receptor binding to 14-3-3 (Chen & Roche 2009)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of mouse brain lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor phosphorylated at Ser¹⁰⁹⁶ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For GRIN2C (Source: Uniprot.org, NCBI)

Alternative Names

glutamate [NMDA] receptor subunit epsilon-3; glutamate receptor, ionotropic, N-methyl D-aspartate 2C; GRIN2C; NMDA R, NR2C Subunit; NMDAR2C; NMDAR2CNR2C; N-methyl D-aspartate receptor subtype 2C; N-methyl-D-aspartate receptor subunit 2C; NR2C GRIN2C GluN2C, NMDAR2C, NR2C glutamate ionotropic receptor NMDA type subunit 2C glutamate receptor ionotropic, NMDA 2C|N-methyl D-aspartate receptor subtype 2C|N-methyl-D-aspartate receptor subunit 2C|glutamate [NMDA] receptor subunit epsilon-3|glutamate receptor, ionotropic, N-methyl D-aspartate 2C

For more info on GRIN2C, check out the GRIN2C Infographic

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In this infographic, you will see the following information for GRIN2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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