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Product Info Summary
| SKU: | A07242 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-NOD3 NLRC3 Antibody
SKU/Catalog Number
A07242
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NOD3 NLRC3 Antibody (Catalog # A07242). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NOD3 NLRC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07242)
Host
Rabbit
Contents
NOD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3. The immunogen is located within amino acids 630 - 680 of NOD3.
Reactive Species
A07242 is reactive to NLRC3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
114.7 kDa
Background of NLRC3
NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-kappaB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07242 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.
Specific Publications For Anti-NOD3 NLRC3 Antibody (A07242)
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