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- Table of Contents
GAR1 antibody (A07049-1) performs well in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of K562 cells using anti-NOLA1/GAR1 antibody (A07049-1).
Overlay histogram showing K562 cells stained with A07049-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOLA1/GAR1 Antibody (A07049-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing K562 cells stained with A07049-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOLA1/GAR1 Antibody (A07049-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-NOLA1/GAR1 Antibody Picoband®
GAR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
GAR1 antibody was positively detected by WB in:
human A375 whole cell, human K562 whole cell, human HL-60 whole cell, human MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GAR1 antibody was positively detected by IHC in:
human breast cancer tissue, human gastric carcinoma tissue, human liver cancer tissue, human ovarian adenoma tissue, human pancreatic cancer tissue, human prostate cancer tissue, human glioma tissue, human lymphoma tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GAR1 antibody was positively detected by ICC/IF in:
MCF-7 cell
GAR1 antibody was positively detected by IP in:
K562 cell
GAR1 antibody was positively detected by FC in:
K562 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NOLA1/GAR1 Antibody Picoband®
View all GAR1 Antibodies
View all GAR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NOLA1/GAR1 recombinant protein (Position: F58-K165).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
25 kDa
Calculated Molecular Weight
22.3 kDa
Background of GAR1
H/ACA ribonucleoprotein complex subunit 1 is a protein that in humans is encoded by the GAR1 gene. This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.
Cite This Product
Anti-NOLA1/GAR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07049-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
99%
High Homology
Sheep
99%
High Homology
Dog
99%
High Homology
Zebrafish
90%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GAR1 antibody
Immunohistochemistry (IHC) protocol for GAR1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GAR1 antibody
Immunoprecipitation (IP) protocol for GAR1 antibody
Flow Cytometry (FC) protocol for GAR1 antibody
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Product Promise
We promise Anti-NOLA1/GAR1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A07049-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026