Anti-Norrin NDP Antibody

Product Name

Anti-Norrin NDP Antibody

View all Norrin/NDP Antibodies

SKU/Catalog Number

A01045

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Norrin NDP Antibody (Catalog # A01045). Tested in ELISA, WB, ICC applications. This antibody reacts with Human.

Storage & Handling

Norrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Norrin NDP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01045)

Host

Rabbit

Contents

Norrin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Norrin antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human Norrin. The immunogen is located within the first 50 amino acids of Norrin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family.

Reactive Species

A01045 is reactive to NDP in Human

Applications

A01045 is guaranteed for ELISA, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

15.044kDa

Background of Norrin/NDP

Norrie disease is an X-linked genetic disorder characterized by progressive atrophy of the eyes, mental disturbances and deafness. The gene responsible for this disease was initially identified through positional cloning. Norrin, the gene product, encodes a small secreted, cysteine-rich protein that is thought to act as a ligand for the Wnt-receptor/beta-catenin signal pathway despite having sequence homology with the Wnt family of proteins. Mice lacking this gene have abnormal blood vessel growth in the vitreous and a disorganized retina; transgenic ectopic expression of Norrin restores normal retinal vasculature. Recent evidence shows that Norrin can attenuate tPA and uPA-mediated death of transformed rat retinal ganglion cells (RGC-5) by activating the Wnt/beta-catenin pathway and regulating the phosphorylation of LRP-1, a cell surface receptor for tPA and uPA, suggesting the Norrin may function in vivo by regulating kinases which may alter the phosphorylation of LRP-1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Norrin antibody can be used for detection of Norrin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Norrin in Jurkat cell lysate with Norrin antibody at (A) 1 and (B) 2 μg/mL.

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Immunocytochemistry of Norrin in Jurkat cells with Norrin antibody at 5 μg/mL.

Gene/Protein Information For NDP (Source: Uniprot.org, NCBI)

Alternative Names

EVR2; exudative vitreoretinopathy 2 (X-linked); FEVR; ND; NDP; Norrie disease (pseudoglioma); Norrie disease protein; Norrin; X-linked exudative vitreoretinopathy 2 protein NDP EVR2, FEVR, ND norrin cystine knot growth factor NDP norrin|NDP, norrin cystine knot growth factor|Norrie disease (pseudoglioma)|X-linked exudative vitreoretinopathy 2 protein|norrie disease protein

For more info on NDP, check out the NDP Infographic

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In this infographic, you will see the following information for NDP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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