Anti-NOTCH 1 Antibody

Product Name

Anti-NOTCH 1 Antibody

View all NOTCH1 Antibodies

SKU/Catalog Number

A00033

Size

200uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NOTCH 1 Antibody (Catalog # A00033). Tested in Dot blot, ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-NOTCH 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00033)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.1% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00033 is reactive to NOTCH1 in Human, Mouse

Applications

A00033 is guaranteed for Dot blot, ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

272.505kDa

Background of NOTCH1

Anti-Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:200
IF Microscopy: User optimized
IP: User optimized
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

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NOTCH 1 was detected in paraffin-embedded sections of exocrine glands of human pancreas using rabbit anti-NOTCH 1 polyclonal antibody (Catalog # A00033) at 1:200. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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Western blot analysis of NOTCH 1 expression in MCF-7 control lysates (lane 1), MCF-7 +1 nM 17-estradiol (lane 2) and MCF-7 + 10

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Western blot analysis of NOTCH 1 expression in Mol wt markers (lane M), no transfection (lane 1), N1 (mouse deleted extracellular domain)-myc (lane 2), N1 (mouse intracellular domain)-myc (lane 3), N2 (mouse deleted extracellular domain)-myc (lane 4), N2 (mouse intracellular domain)-myc (lane 5), N3 (mouse deleted extracellular domain)-myc (lane 6), N3 (mouse intracellular domain)-myc (lane 7), N4 (mouse deleted extracellular domain)-myc (lane 8), N4 (mouse intracellular domain)-myc (lane 9) and N1 (mouse deleted extracellular domain)(V to G)-myc (lane 10). NOTCH 1 was detected using rabbit anti-NOTCH 1 polyclonal antibody (Catalog # A00033) at 1:500. Personal communication, Dr. Stacey Huppert.

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Dot Blot of Rabbit anti-NOTCH 1 polyclonal antibody. Antigen: Row 1 -Notch 1 Peptide (Cleaved N Terminal) Row 2 -Notch 1 (Intra) Peptide. Load: Lane 1 -200 ng Lane 2 -66.67 ng Lane 3 -22.22 ng Lane 4 -7.41 ng Lane 5 -2.47 ng. Primary antibody: Rabbit anti-Notch 1 (Cleaved N Terminal) (Human Specific) Antibody at 1:1000 for 60 min at RT. Secondary antibody: HRP Rabbit Secondary at 1:40000 for 30 min at RT. Block: MB-070 for 1 HR at RT.

Gene/Protein Information For NOTCH1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 NOTCH1 AOS5, AOVD1, TAN1, hN1 notch receptor 1 neurogenic locus notch homolog protein 1|Notch homolog 1, translocation-associated|notch 1|translocation-associated notch protein TAN-1

For more info on NOTCH1, check out the NOTCH1 Infographic

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In this infographic, you will see the following information for NOTCH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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