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Product Info Summary
| SKU: | M00403 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NOX4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00403
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NOX4 Rabbit Monoclonal Antibody catalog # M00403. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOX4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00403)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
E-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NOX4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00403 is reactive to Nox4 in Human, Mouse, Rat
Applications
M00403 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
66.519kDa
Background of Nox4
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HeLa cells, using NOX4 Antibody .
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Western blot analysis of NOX4 expression in JAR cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded rat kidney, using NOX4 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For Nox4 (Source: Uniprot.org, NCBI)
Gene Name
Nox4
Full Name
NADPH oxidase 4
Weight
66.519kDa
Alternative Names
EC 1.6.3; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX; KOX-1; KOX-1Renal NAD(P)H-oxidase; NADPH oxidase 4; Nox4; RENOX; RENOXEC 1.6.3.- Nox4|AI648021|NADPH oxidase 4|NADPH oxidase 4|KOX-1|kidney oxidase-1|kidney superoxide-producing NADPH oxidase|renal NAD(P)H-oxidase|superoxide-generating NADPH oxidase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nox4, check out the Nox4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nox4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOX4 Rabbit Monoclonal Antibody (M00403)
Hello CJ!
M00403 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang D,Yan Z,Bu L,An C,Deng B,Zhang J,Rao J,Cheng L,Zhang J,Zhang B,Xie J.Protective effect of peptide DR8 on bleomycin-induced pulmonary fibrosis by regulating the TGF-β/MAPK signaling pathway and oxidative stress.Toxicol Appl Pharmacol.2019 Nov 1;382:114703.doi:10.1016/j.taap.2019.114703.Epub 2019 Aug 6.PMID:31398421.
Species: Mouse
M00403 usage in article: APP:WB, SAMPLE:NIH3T3 CELL AND A549 CELL, DILUTION:1:200
Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation
Obligatory role for GPER in cardiovascular aging and disease^
Zhao, X., Li, R., Liu, Y., Zhang, X., Zhang, M., Zeng, Z.,…, & Wang, Y. (2017). Polydatin protects against carbon tetrachloride-induced liver fibrosis in mice. Archives of Biochemistry and Biophysics, 629, 1-7. Advance online publication. doi: 10....
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
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18 Customer Q&As for Anti-NOX4 Rabbit Monoclonal Antibody
Question
We are currently using anti-NOX4 Rabbit Monoclonal antibody M00403 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The anti-NOX4 Rabbit Monoclonal antibody (M00403) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
Question
I see that the anti-NOX4 Rabbit Monoclonal antibody M00403 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-20
Question
Does this antibody recognize Nox4D?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The immunogen sequence for M00403 corresponds to Human NADPH oxidase 4 from Q500 to S578. On the basis of sequence information, M00403 is able to recognize Nox4D.
Boster Scientific Support
Answered: 2019-12-26
Question
My question regarding product M00403, anti-NOX4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00403 anti-NOX4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-02
Question
I am interested in to test anti-NOX4 Rabbit Monoclonal antibody M00403 on rat ovary for research purposes, then I may be interested in using anti-NOX4 Rabbit Monoclonal antibody M00403 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The products we sell, including anti-NOX4 Rabbit Monoclonal antibody M00403, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-NOX4 Rabbit Monoclonal antibody M00403. Let me know if you need anything else.
L. Gonzalez
Verified customer
Asked: 2019-09-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
We were well pleased with the WB result of your anti-NOX4 Rabbit Monoclonal antibody. However we have seen positive staining in kidney endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is NOX4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
According to literature, kidney does express NOX4. Generally NOX4 expresses in endoplasmic reticulum membrane. Regarding which tissues have NOX4 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Pubmed ID: 11376945
Kidney, Pubmed ID: 10869423, 11032835
Lung, Pubmed ID: 15721269
Ovary, Pubmed ID: 15489334
Pulmonary artery, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-21
Question
Would anti-NOX4 Rabbit Monoclonal antibody M00403 work on horse ICC with ovary?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
Our lab technicians have not tested anti-NOX4 Rabbit Monoclonal antibody M00403 on horse. You can run a BLAST between horse and the immunogen sequence of anti-NOX4 Rabbit Monoclonal antibody M00403 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse ovary in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Do you have a BSA free version of anti-NOX4 Rabbit Monoclonal antibody M00403 available?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NOX4 Rabbit Monoclonal antibody M00403 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-22
Question
We need using your anti-NOX4 Rabbit Monoclonal antibody for detoxification of reactive oxygen species studies. Has this antibody been tested with western blotting on rat kidney? We would like to see some validation images before ordering.
C. Huang
Verified customer
Asked: 2018-08-02
Answer
Thank you for your inquiry. This M00403 anti-NOX4 Rabbit Monoclonal antibody is validated on rat kidney, hela cells. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-02
Question
Our lab used your anti-NOX4 Rabbit Monoclonal antibody for IF on nephron tubule in a previous project. I am using rat, and I plan to use the antibody for IHC next. We are interested in examining nephron tubule as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
I viewed the website and datasheets of our anti-NOX4 Rabbit Monoclonal antibody and it seems that M00403 has been validated on rat in both IF and IHC. Thus M00403 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-09
Question
We have been able to see staining in mouse fetal kidney. Do you have any suggestions? Is anti-NOX4 Rabbit Monoclonal antibody supposed to stain fetal kidney positively?
D. Carter
Verified customer
Asked: 2017-12-04
Answer
According to literature fetal kidney does express NOX4. According to Uniprot.org, NOX4 is expressed in nephron tubule, kidney, fetal kidney, lung, pulmonary artery, ovary, among other tissues. Regarding which tissues have NOX4 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Pubmed ID: 11376945
Kidney, Pubmed ID: 10869423, 11032835
Lung, Pubmed ID: 15721269
Ovary, Pubmed ID: 15489334
Pulmonary artery, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-12-04
Question
Would anti-NOX4 Rabbit Monoclonal antibody M00403 work for IP with ovary?
F. Bhatt
Verified customer
Asked: 2017-04-18
Answer
According to the expression profile of ovary, NOX4 is highly expressed in ovary. So, it is likely that anti-NOX4 Rabbit Monoclonal antibody M00403 will work for IP with ovary.
Boster Scientific Support
Answered: 2017-04-18
Question
I was wanting to use your anti-NOX4 Rabbit Monoclonal antibody for IP for rat ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat ovary identification?
J. Lewis
Verified customer
Asked: 2016-12-28
Answer
You can see on the product datasheet, M00403 anti-NOX4 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-28
Question
Here is the WB image, lot number and protocol we used for ovary using anti-NOX4 Rabbit Monoclonal antibody M00403. Please let me know if you require anything else.
A. Zhao
Verified customer
Asked: 2016-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-25
Question
Is a blocking peptide available for product anti-NOX4 Rabbit Monoclonal antibody (M00403)?
D. Jha
Verified customer
Asked: 2016-01-07
Answer
We do provide the blocking peptide for product anti-NOX4 Rabbit Monoclonal antibody (M00403). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-07
Question
Does M00403 anti-NOX4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Johnson
Verified customer
Asked: 2015-07-24
Answer
As indicated on the product datasheet, M00403 anti-NOX4 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-24
Question
Is this M00403 anti-NOX4 Rabbit Monoclonal antibody reactive to the isotypes of NOX4?
B. Brown
Verified customer
Asked: 2015-03-27
Answer
The immunogen of M00403 anti-NOX4 Rabbit Monoclonal antibody is A synthesized peptide derived from human NOX4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-27
