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Product Info Summary
| SKU: | A02476-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-NOXA2/p67phox/NCF2 Antibody Picoband™
View all p67phox/NOXA2 Antibodies
SKU/Catalog Number
A02476-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NOXA2/p67phox/NCF2 Antibody Picoband™ catalog # A02476-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOXA2/p67phox/NCF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02476-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NOXA2/p67phox/NCF2 recombinant protein (Position: M1-V526).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02476-1 is reactive to NCF2 in Human, Mouse, Rat
Applications
A02476-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
59.762kDa
Background of p67phox/NOXA2
This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA2/p67phox/NCF2 antigen affinity purified polyclonal antibody (Catalog # A02476-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA2/p67phox/NCF2 at approximately 60KD. The expected band size for NOXA2/p67phox/NCF2 is at 60KD.
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Figure 2. IHC analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1).
NOXA2/p67phox/NCF2 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOXA2/p67phox/NCF2 Antibody (A02476-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1).
NOXA2/p67phox/NCF2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOXA2/p67phox/NCF2 Antibody (A02476-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1).
NOXA2/p67phox/NCF2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOXA2/p67phox/NCF2 Antibody (A02476-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of THP-1 cells using anti-NOXA2/p67phox/NCF2 antibody (A02476-1).
Overlay histogram showing THP-1 cells stained with A02476-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOXA2/p67phox/NCF2 Antibody (A02476-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NCF2 (Source: Uniprot.org, NCBI)
Gene Name
NCF2
Full Name
Neutrophil cytosol factor 2
Weight
59.762kDa
Superfamily
NCF2/NOXA1 family
Alternative Names
autosomal 2; NADPH oxidase activator 2; NCF2; NCF-2; neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal2); neutrophil cytosolic factor 2; NOXA2; NOXA2FLJ93058; p67phox; p67-phox NCF2 NCF-2, NOXA2, P67-PHOX, P67PHOX neutrophil cytosolic factor 2 neutrophil cytosol factor 2|67 kDa neutrophil oxidase factor|NADPH oxidase activator 2|chronic granulomatous disease, autosomal 2|neutrophil NADPH oxidase factor 2|neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCF2, check out the NCF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOXA2/p67phox/NCF2 Antibody Picoband™ (A02476-1)
Hello CJ!
A02476-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Sildenafil improves diabetic vascular activity through suppressing endothelin receptor A, iNOS and NADPH oxidase which is comparable with the endothelin receptor antagonist CPU0213 in STZ‐injected rats
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