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Product Info Summary
| SKU: | A14044 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NSA1 WDR74 Antibody
SKU/Catalog Number
A14044
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NSA1 WDR74 Antibody (Catalog # A14044). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NSA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NSA1 WDR74 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14044)
Host
Rabbit
Contents
NSA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NSA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NSA1. The immunogen is located within amino acids 320 - 370 of NSA1.
Reactive Species
A14044 is reactive to WDR74 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.4 kDa
Background of WDR74
The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14044 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NSA1 in mouse liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NSA1 in rat liver tissue with NSA1 antibody at 5 μg/mL.
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Immunofluorescence of NSA1 in rat liver tissue with NSA1 antibody at 20 μg/mL.
Specific Publications For Anti-NSA1 WDR74 Antibody (A14044)
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