Anti-NTAL/LAT2 Antibody Picoband™

Product Name

Anti-NTAL/LAT2 Antibody Picoband™

View all NTAL Antibodies

SKU/Catalog Number

A02496-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NTAL/LAT2 Antibody Picoband™ catalog # A02496-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NTAL/LAT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02496-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NTAL/LAT2 recombinant protein (Position: R46-S217).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02496-1 is reactive to LAT2 in Human

Applications

A02496-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

26.55kDa

Background of NTAL

Linker for activation of T-cells family member 2, also known as WBSCR5 or NTAL is a protein that in humans is encoded by the LAT2 gene. It is mapped to 7q11.23. It has been found that NTAL associated with GRB2, SOS1 and GAB1 after B-cell receptor activation. Phosphorylation of NTAL was strongest in the presence of LCK, ZAP70, SYK, or LYNLAT2. NTAL is a structural and possibly a functional homolog of LAT in non-T cells. It also has been found that BLNK and LAT2 in B cells are a functionally equivalent pair to SLP76 and LAT in T cells. This gene consists of at least 14 exons, and its alternative splicing generates 3 transcript variants, all encoding the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of NTAL/LAT2 using anti-NTAL/LAT2 antibody (A02496-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTAL/LAT2 antigen affinity purified polyclonal antibody (Catalog # A02496-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTAL/LAT2 at approximately 27 kDa. The expected band size for NTAL/LAT2 is at 27 kDa.

Gene/Protein Information For LAT2 (Source: Uniprot.org, NCBI)

Alternative Names

LAB; LABWilliams-Beuren syndrome chromosomal region 15 protein; Linker for activation of B-cells; linker for activation of T cells family, member 2; linker for activation of T-cells family member 2; Membrane-associated adapter molecule; Non-T-cell activation linker; NTAL; NTALWilliams-Beuren syndrome chromosomal region 5 protein; WBS15; WBSCR15; WBSCR5; WBSCR5HSPC046; Williams-Beuren syndrome chromosome region 5; WSCR5 LAT2 HSPC046, LAB, NTAL, WBSCR15, WBSCR5, WSCR5 linker for activation of T cells family member 2 linker for activation of T-cells family member 2|Williams-Beuren syndrome chromosomal region 15 protein|Williams-Beuren syndrome chromosomal region 5 protein|linker for activation of B-cells|linker for activation of T cells, transmembrane adaptor 2|membrane-associated adapter molecule|non-T-cell activation linker

For more info on LAT2, check out the LAT2 Infographic

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In this infographic, you will see the following information for LAT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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