Anti-Nucleobindin-2 NUCB2 Antibody

Nesfatin-1/Nucleobindin-2 antibody

Boster Bio Anti-Nucleobindin-2 NUCB2 Antibody (Catalog # A02400). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02400
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, WB

Product Name

Anti-Nucleobindin-2 NUCB2 Antibody

View all Nesfatin-1/Nucleobindin-2 Antibodies

SKU/Catalog Number

A02400

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Nucleobindin-2 NUCB2 Antibody (Catalog # A02400). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Nucleobindin-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Nucleobindin-2 NUCB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02400)

Host

Rabbit

Contents

Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 . The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.

Reactive Species

A02400 is reactive to NUCB2 in Human, Mouse, Rat

Applications

A02400 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.223kDa

Background of Nesfatin-1/Nucleobindin-2

NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For NUCB2 (Source: Uniprot.org, NCBI)

Gene Name

NUCB2

Full Name

Nucleobindin-2

Weight

50.223kDa

Superfamily

nucleobindin family

Alternative Names

DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2 NUCB2 HEL-S-109, NEFA nucleobindin 2 nucleobindin-2|DNA-binding protein NEFA|epididymis secretory protein Li 109|gastric cancer antigen Zg4|nesfatin 1|novel DNA binding/EF-hand/leucine zipper protein|nucleobinding 2|prepronesfatin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NUCB2, check out the NUCB2 Infographic

NUCB2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NUCB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A02400-0.1 mg

0.1 mg

$449

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00

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