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Product Info Summary
| SKU: | A02400 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Nucleobindin-2 NUCB2 Antibody
SKU/Catalog Number
A02400
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Nucleobindin-2 NUCB2 Antibody (Catalog # A02400). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Nucleobindin-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Nucleobindin-2 NUCB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02400)
Host
Rabbit
Contents
Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 . The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.
Cross-reactivity
Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.
Reactive Species
A02400 is reactive to NUCB2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.2 kDa
Background of NUCB2
NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02400 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.
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Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.
Specific Publications For Anti-Nucleobindin-2 NUCB2 Antibody (A02400)
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