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Product Info Summary
| SKU: | M14439 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-OPAL1 Purified WBP1L Monoclonal Antibody
SKU/Catalog Number
M14439
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-OPAL1 Purified WBP1L Monoclonal Antibody (Catalog# M14439). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-OPAL1 Purified WBP1L Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14439)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
OPAL1-01
Isotype
Mouse IgG2a
Immunogen
Recombinant fragment of human OPAL1 (amino acids 152-342). The mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (77), alpha-hCG (1.3), hLH (0.86), hFSH (< 0.5), hTSH (< 0.5).
Reactive Species
M14439 is reactive to WBP1L in Human
Applications
M14439 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
37.828kDa
Background of OPAL1
OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml, intracellular staining.
Western blotting: 1-2 μg/ml, non-reducing conditions.
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry validation of WBP1L using Anti-OPAL1 Purified WBP1L Monoclonal Antibody (M14439).
Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.
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Figure 1. Western blotting validation for Anti-OPAL1 Purified WBP1L Monoclonal Antibody M14439
Western blotting analysis of OPAL1 in OPAL1-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For WBP1L (Source: Uniprot.org, NCBI)
Gene Name
WBP1L
Full Name
WW domain binding protein 1-like
Weight
37.828kDa
Alternative Names
chromosome 10 open reading frame 26; FLJ20154; FLJ20367; MGC125648; OPA1L; OPAL1; outcome predictor in acute leukemia 1 WBP1L C10orf26, OPA1L, OPAL1 WW domain binding protein 1 like WW domain binding protein 1-like|outcome predictor in acute leukemia 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WBP1L, check out the WBP1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WBP1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OPAL1 Purified WBP1L Monoclonal Antibody (M14439)
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No publications found for M14439
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