|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-p107 Antibody|
|Description||Rabbit IgG polyclonal antibody for Retinoblastoma-like protein 1(RBL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-p107 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1894)|
|Replacement Item||This antibody may replace the following items: sc-53966|sc-20081|sc-74589|sc-53965|sc-32022|sc-32021|sc-59157|sc-52660|sc-65740|sc-11038|sc-53967|sc-53580|sc-65892|sc-23926 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human p107(1048-1068aa QENDDVLLKRLQDVVSERANH), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Retinoblastoma-like protein 1|
|Molecular Weight||120847 MW|
|Protein Function||Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.|
|Subcellular Localization||Nucleus .|
|Alternative Names||Retinoblastoma-like protein 1;107 kDa retinoblastoma-associated protein;p107;pRb1;RBL1;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| stem cells|signaling pathways|tgf beta|nuclear| cancer|oncoproteins/suppressors|rb family||
Background for Retinoblastoma-like protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-p107 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-p107 Antibody Images
Click the images to enlarge.
Lane 1: 239T Cell Lysate
Lane 2: JURKAT Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Rat Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,