Anti-p107/RBL1 Antibody

Product Name

Anti-p107/RBL1 Antibody

View all p107 Antibodies

SKU/Catalog Number

PA1894

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p107/RBL1 Antibody catalog # PA1894. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p107/RBL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1894)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human p107, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1894 is reactive to RBL1 in Human, Mouse, Rat

Applications

PA1894 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

120.847kDa

Background of p107

RBL1 (Retinoblastoma-like 1), also known as Cullular Proteins p107, CP107 and 107, is a protein that in humans is encoded by the RBL1 gene. Ewen et al. (1991)used a partial cDNA for human p107 to map the gene to 20q11. by fluorescence in situ hybridization. Huppi et al. (1996)found that the mouse Rbl1 gene maps to the distal end of chromosome 2, as does also the E2f1 gene. The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is though that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Anti-p107 antibody, PA1894, Western blotting
Lane 1: 239T Cell Lysate
Lane 2: JURKAT Cell Lysate

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Anti-p107 antibody, PA1894, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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Anti-p107 antibody, PA1894, IHC(P)
IHC(P): Rat Intestine Tissue

Gene/Protein Information For RBL1 (Source: Uniprot.org, NCBI)

Alternative Names

cellular protein 107,107 kDa retinoblastoma-associated protein; cp107; p107MGC40006; PRB1; retinoblastoma-like 1 (p107); retinoblastoma-like protein 1 RBL1 CP107, PRB1, p107 RB transcriptional corepressor like 1 retinoblastoma-like protein 1|107 kDa retinoblastoma-associated protein|retinoblastoma-like 1

For more info on RBL1, check out the RBL1 Infographic

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In this infographic, you will see the following information for RBL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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