Anti-p16 CDKN2A Monoclonal Antibody

Boster Bio Anti-p16 CDKN2A Monoclonal Antibody catalog # A00016-3. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00016-3
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: ELISA, IHC-P, WB

Product Name

Anti-p16 CDKN2A Monoclonal Antibody

See all CDKN2A products

SKU/Catalog Number

A00016-3

Size

100μl

Form

Liquid

Description

Boster Bio Anti-p16 CDKN2A Monoclonal Antibody catalog # A00016-3. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p16 CDKN2A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00016-3)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.

Clone Number

1E12E1

Isotype

IgG1

Immunogen

Purified recombinant fragment of p16 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00016-3 is reactive to CDKN2A in Human, Mouse, Rat

Applications

A00016-3 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA 1:10000
IHC 1:200-1:1000
WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For CDKN2A (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8N726

Gene ID

1029

Gene Name

CDKN2A

Full Name

Tumor suppressor ARF

Weight

13.903kDa

Alternative Names

alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16 cyclin dependent kinase inhibitor 2A cyclin-dependent kinase inhibitor 2A|CDK4 inhibitor p16-INK4|alternative reading frame|cell cycle negative regulator beta|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|multiple tumor suppressor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDKN2A, check out the CDKN2A Infographic

CDKN2A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDKN2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00016-3 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of p16 protein in infantile hemangioma

Song Lj, Liu Rj, Zeng Z, Alper Sl, Cui Hj, Lu Y, Zheng L, Yan Zw, Fu Gh. J Mol Med (Berl). 2012 Jun;90(6):707-18. Doi: 10.1007/S00109-011-0851-2. Epub 2012 Jan 7. Gastrin Inhibits A Novel, Pathological Colon Cancer Signaling Pathway Involving Egr1...

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12 Customer Q&As for Anti-p16 CDKN2A Monoclonal Antibody

Question

My question regards to test anti-p16 Monoclonal antibody A00016-3 on rat adenohypophysis for research purposes, then I may be interested in using anti-p16 Monoclonal antibody A00016-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

The products we sell, including anti-p16 Monoclonal antibody A00016-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-05-08

Question

Is there a BSA free version of anti-p16 Monoclonal antibody A00016-3 available?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16 Monoclonal antibody A00016-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-09

Question

Does A00016-3 anti-p16 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

As indicated on the product datasheet, A00016-3 anti-p16 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-02

Question

Will anti-p16 Monoclonal antibody A00016-3 work for IHC with adenohypophysis?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16 Monoclonal antibody A00016-3 will work for IHC with adenohypophysis.

Boster Scientific Support

Answered: 2020-01-03

Question

I see that the anti-p16 Monoclonal antibody A00016-3 works with IHC, what is the protocol used to produce the result images on the product page?

M. Mangal

Verified customer

Asked: 2019-12-09

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-09

Question

Is this A00016-3 anti-p16 Monoclonal antibody reactive to the isotypes of CDKN2A?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

The immunogen of A00016-3 anti-p16 Monoclonal antibody is Purified recombinant fragment of p16 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-30

Question

Is a blocking peptide available for product anti-p16 Monoclonal antibody (A00016-3)?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

We do provide the blocking peptide for product anti-p16 Monoclonal antibody (A00016-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-24

Question

My question regarding product A00016-3, anti-p16 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00016-3 anti-p16 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-28

Question

See below the WB image, lot number and protocol we used for adenohypophysis using anti-p16 Monoclonal antibody A00016-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-14

Question

I was wanting to use your anti-p16 Monoclonal antibody for IHC for rat adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adenohypophysis identification?

Verified Customer

Verified customer

Asked: 2018-03-26

Answer

You can see on the product datasheet, A00016-3 anti-p16 Monoclonal antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adenohypophysis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-26

Question

We are currently using anti-p16 Monoclonal antibody A00016-3 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

T. Johnson

Verified customer

Asked: 2018-02-09

Answer

The anti-p16 Monoclonal antibody (A00016-3) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-09

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16 Monoclonal antibody A00016-3. Let me know if you need anything else.

D. Taylor

Verified customer

Asked: 2013-11-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-22

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A00016-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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