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Product Info Summary
| SKU: | A00016 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p16 Cdkn2a Antibody
SKU/Catalog Number
A00016
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-p16 Cdkn2a Antibody (Catalog # A00016). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-p16 Cdkn2a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00016)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
human p16 protein (missing the first 7 amino acids)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00016 is reactive to CDKN2A in Human
Applications
A00016 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
16.533kDa
Background of CDKN2A
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene (isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:500
IP: 1:100
WB: 1:500 - 1:5,000
Validation Images & Assay Conditions
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Anti-p16 antibody was diluted 1:500 to detect p16 in human prostate tissue.Tissue was formalin fixed and paraffin embedded.No pre-treatment of sample was required.The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Protein Target Info & Infographic
Gene/Protein Information For CDKN2A (Source: Uniprot.org, NCBI)
Gene Name
CDKN2A
Full Name
Cyclin-dependent kinase inhibitor 2A
Weight
16.533kDa
Superfamily
CDKN2 cyclin-dependent kinase inhibitor family
Alternative Names
alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16 cyclin dependent kinase inhibitor 2A cyclin-dependent kinase inhibitor 2A|CDK4 inhibitor p16-INK4|alternative reading frame|cell cycle negative regulator beta|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|multiple tumor suppressor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN2A, check out the CDKN2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p16 Cdkn2a Antibody (A00016)
Hello CJ!
A00016 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation
Expression of p16 protein in infantile hemangioma
Recommended Resources
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-p16 Cdkn2a Antibody
Question
Can you help my question with product A00016, anti-p16 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00016 anti-p16 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-21
Question
Does anti-p16 antibody A00016 work for IP with adenohypophysis?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16 antibody A00016 will work for IP with adenohypophysis.
Boster Scientific Support
Answered: 2020-01-28
Question
I was wanting to use your anti-p16 antibody for IP for human adenohypophysis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can see on the product datasheet, A00016 anti-p16 antibody has been validated for IP on human tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
See attached the WB image, lot number and protocol we used for adenohypophysis using anti-p16 antibody A00016. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16 antibody A00016. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-p16 antibody A00016 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
The anti-p16 antibody (A00016) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-02
Question
Do you have a BSA free version of anti-p16 antibody A00016 available?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-p16 antibody A00016 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-18
Question
Is this A00016 anti-p16 antibody reactive to the isotypes of CDKN2A?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
The immunogen of A00016 anti-p16 antibody is human p16 protein (missing the first 7 amino acids) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-30
Question
I see that the anti-p16 antibody A00016 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-31
Question
I am interested in to test anti-p16 antibody A00016 on human adenohypophysis for research purposes, then I may be interested in using anti-p16 antibody A00016 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Zhao
Verified customer
Asked: 2016-10-04
Answer
The products we sell, including anti-p16 antibody A00016, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-10-04
Question
Would A00016 anti-p16 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Jones
Verified customer
Asked: 2015-03-25
Answer
It shows on the product datasheet, A00016 anti-p16 antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-25
Question
Is a blocking peptide available for product anti-p16 antibody (A00016)?
J. Anderson
Verified customer
Asked: 2014-01-01
Answer
We do provide the blocking peptide for product anti-p16 antibody (A00016). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-01-01
