Click image to see more details
Product Info Summary
| SKU: | M02096 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02096
BM4192 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody catalog # M02096. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02096)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CCD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p16 ARC
Reactive Species
M02096 is reactive to ARPC5 in Human, Mouse, Rat
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02096 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human THP-1 whole cell, human Hela whole cell, human U251 whole cell, rat spleen tissue, rat C6 whole cell, mouse spleen tissue, mouse Neuro-2a whole cell
IHC: human colon tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ARPC5 using anti-ARPC5 antibody (M02096).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARPC5 antigen affinity purified monoclonal antibody (M02096) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARPC5 at approximately 16 kDa. The expected band size for ARPC5 is at 16 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using p16 ARC Antibody.
Specific Publications For Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody (M02096)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-p16 ARC ARPC5 Rabbit Monoclonal Antibody
Question
Will anti-p16 ARC Rabbit Monoclonal antibody M02096 work for IP with platelet?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
According to the expression profile of platelet, ARPC5 is highly expressed in platelet. So, it is likely that anti-p16 ARC Rabbit Monoclonal antibody M02096 will work for IP with platelet.
Boster Scientific Support
Answered: 2020-04-23
Question
Is a blocking peptide available for product anti-p16 ARC Rabbit Monoclonal antibody (M02096)?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
We do provide the blocking peptide for product anti-p16 ARC Rabbit Monoclonal antibody (M02096). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-p16 ARC Rabbit Monoclonal antibody M02096 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The anti-p16 ARC Rabbit Monoclonal antibody (M02096) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-20
Question
I was wanting to use your anti-p16 ARC Rabbit Monoclonal antibody for IP for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can see on the product datasheet, M02096 anti-p16 ARC Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Can you confirm if M02096 works well in Immunocytochemistry and Immunofluorescence?
Verified customer
Asked: 2019-03-27
Answer
The Anti-P16 ARC ARPC5 Rabbit Monoclonal Antibody (M02096) has been validated for ICC and IF application.
Boster Scientific Support
Answered: 2019-03-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-p16 ARC Rabbit Monoclonal antibody M02096. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-18
