Anti-p16 INK Monoclonal Antibody

Product Name

Anti-p16 INK Monoclonal Antibody

View all CDKN2A Antibodies

SKU/Catalog Number

M00016

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-p16 INK Monoclonal Antibody catalog # M00016. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p16 INK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00016)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CGD-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human p16 INK

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00016 is reactive to CDKN2A in Human

Applications

M00016 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

16.533kDa

Background of CDKN2A

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:100

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Western blot analysis of p16 INK expression in 293T cell lysate.

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded human gastric, using p16 INK Antibody.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)

Gene/Protein Information For CDKN2A (Source: Uniprot.org, NCBI)

Alternative Names

alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16 cyclin dependent kinase inhibitor 2A cyclin-dependent kinase inhibitor 2A|CDK4 inhibitor p16-INK4|alternative reading frame|cell cycle negative regulator beta|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|multiple tumor suppressor 1

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