Product Info Summary
SKU: | A01208-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-P2X7/P2RX7 Antibody Picoband™
View all P2X7/P2RX7 Antibodies
SKU/Catalog Number
A01208-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P2X7/P2RX7 Antibody Picoband™ catalog # A01208-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-P2X7/P2RX7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01208-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human P2X7/P2RX7 recombinant protein (Position: S47-K311).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01208-3 is reactive to P2RX7 in Human, Mouse, Rat
Applications
A01208-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
68.585kDa
Background of P2X7/P2RX7
P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of P2X7/P2RX7 using anti-P2X7/P2RX7 antibody (A01208-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X7/P2RX7 antigen affinity purified polyclonal antibody (Catalog # A01208-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X7/P2RX7 at approximately 70 kDa. The expected band size for P2X7/P2RX7 is at 70 kDa.
Click image to see more details
Figure 2. IHC analysis of P2X7/P2RX7 using anti-P2X7/P2RX7 antibody (A01208-3).
P2X7/P2RX7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P2X7/P2RX7 Antibody (A01208-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of P2X7/P2RX7 using anti-P2X7/P2RX7 antibody (A01208-3).
P2X7/P2RX7 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinomar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P2X7/P2RX7 Antibody (A01208-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of P2X7/P2RX7 using anti-P2X7/P2RX7 antibody (A01208-3).
P2X7/P2RX7 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P2X7/P2RX7 Antibody (A01208-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of THP-1 cells using anti-P2X7/P2RX7 antibody (A01208-3).
Overlay histogram showing THP-1 cells stained with A01208-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P2X7/P2RX7 Antibody (A01208-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For P2RX7 (Source: Uniprot.org, NCBI)
Gene Name
P2RX7
Full Name
P2X purinoceptor 7
Weight
68.585kDa
Superfamily
P2X receptor family
Alternative Names
ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7P2X7 receptor; P2X7R; P2Z receptor; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A; Purinergic receptor P2RX7 P2X7 purinergic receptor P2X 7 P2X purinoceptor 7|ATP receptor|P2X7 receptor|P2Z receptor|purinergic receptor P2X, ligand gated ion channel, 7|purinergic receptor P2X7 variant A|purnergic receptor P2X 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P2RX7, check out the P2RX7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P2RX7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P2X7/P2RX7 Antibody Picoband™ (A01208-3)
Hello CJ!
A01208-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased expression of P2X7 receptor in peripheral blood mononuclear cells correlates with clinical severity and serum levels of Th17-related cytokines in patients with myasthenia gravis
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