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MAPK14 antibody (A00176-2) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 29 Publications

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a00176 2 mapk14 primary antibodies fcm testing 6

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Flow Cytometry analysis of CACO-2 cells using anti-P38 Alpha/MAPK14 antibody (A00176-2).
Overlay histogram showing CACO-2 cells stained with A00176-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P38 Alpha/MAPK14 Antibody (A00176-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-p38 alpha/MAPK14 Antibody Picoband®

MAPK14 antibody

29 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

MAPK14 antibody was positively detected by WB in:

human Jurkat whole cell, human SW620 whole cell, human Hela whole cell, human CACO-2 whole cell, human Hela- WT whole cell, human Hela-MAPK14 KO whole cell, mouse spleen tissue, mouse RAW264.7 whole cell, rat lung tissue, rat heart tissue, mouse lung tissue, mouse heart tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MAPK14 antibody was positively detected by IHC in:

human breast cancer tissue, mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MAPK14 antibody was positively detected by ICC/IF in:

Hela cell

MAPK14 antibody was positively detected by IP in:

Jurkat cell

MAPK14 antibody was positively detected by FC in:

CACO-2 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Mouse

Tested

Recommend

Rat

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-p38 alpha/MAPK14 Antibody Picoband®
View all MAPK14 Antibodies

Form

Lyophilized

Uniprot

Q16539

Gene ID

1432

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human p38 alpha/MAPK14, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

38 kDa

Calculated Molecular Weight

41.3 kDa

Background of MAPK14

Mitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Cite This Product

Anti-p38 alpha/MAPK14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00176-2)

Protocols

Western Blot protocol for MAPK14 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MAPK14 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MAPK14 antibody

Review Protocol

Immunoprecipitation (IP) protocol for MAPK14 antibody

Review Protocol

Flow Cytometry (FC) protocol for MAPK14 antibody

Review Protocol

Publications

In-Depth Analysis of AG73-Dental Pulp Cell Interactions Underlying Adhesion and Mineralization: Insights from Alanine Scanning

Published: 2026-07-01

Journal: Tissue Engineering and Regenerative Medicine

Application: WB

Impact Factor: 5.10

Species: Human

PMID: 42384314

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Anti-p38 alpha/MAPK14 Antibody Picoband®

MAPK14 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

In-Depth Analysis of AG73-Dental Pulp Cell Interactions Underlying Adhesion and Mineralization: Insights from Alanine Scanning

Anti-asthmatic effects and ingredient annotation of the active fraction from Ganoderma lucidum

A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization

Investigating the Mechanism of the Fuzheng Huayu Formula in Treating Cirrhosis through Network Pharmacology, Molecular Docking, and Experimental Verification

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX-2 signaling

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

Dioscin induces osteosarcoma cell apoptosis by upregulating ROS-mediated P38 MAPK signaling

Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism

Computational and In Vitro Analysis of Plumbagin’s Molecular Mechanism for the Treatment of Hepatocellular Carcinoma

Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential

Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways

Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier

Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro

The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells

Dehydroepiandrosterone Prevents H2O2-Induced BRL-3A Cell Oxidative Damage through Activation of PI3K/Akt Pathways rather than MAPK Pathways

Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats

Lower range of molecular weight of xanthan gum inhibits apoptosis of chondrocytes through MAPK signaling pathways

Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways

The impact of acute thermal stress on green mussel Perna viridis: Oxidative damage and responses

Let-7d microRNA Attenuates 6-OHDA-Induced Injury by Targeting Caspase-3 in MN9D Cells

Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response

β-d-glucan from Antrodia Camphorata ameliorates LPS-induced inflammation and ROS production in human hepatocytes

The vascular protective effects of Anoectochilus roxburghii polysaccharose under high glucose conditions

Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells

Altholactone induces reactive oxygen species-mediated apoptosis in bladder cancer T24 cells through mitochondrial dysfunction, MAPK-p38 activation and Akt suppression

Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation

Panax Notoginseng Saponins Promote Osteogenic Differentiation of Bone Marrow Stromal Cells Through the ERK and P38 MAPK Signaling Pathways

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