Product Info Summary
SKU: | A05917 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-P4HA1 Antibody
SKU/Catalog Number
A05917
Size
100ul
Form
Liquid
Description
Boster Bio Anti-P4HA1 Antibody catalog # A05917. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P4HA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05917)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PGLYRP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05917 is reactive to P4HA1 in Human, Mouse, Rat
Applications
A05917 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
61.049kDa
Background of P4HA1
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
Chen D., Gene 174:129-134(1996) [PubMed: 8863739].
Bao X.,Eur. J. Biochem. 269:259-271(2002) [PubMed: 11784320].
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-P4HA1 Antibody A05917
Western blot analysis of extracts of various cellsProtein Target Info & Infographic
Gene/Protein Information For P4HA1 (Source: Uniprot.org, NCBI)
Gene Name
P4HA1
Full Name
Prolyl 4-hydroxylase subunit alpha-1
Weight
61.049kDa
Superfamily
P4HA family
Alternative Names
2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); 2-oxoglutarate-4-dioxygenase subunit alpha-1; 4-PH alpha-1; alpha polypeptide I; EC 1.14.11.2; Procollagen-proline; prolyl 4-hydroxylase, alpha polypeptide I P4HA1 P4HA prolyl 4-hydroxylase subunit alpha 1 prolyl 4-hydroxylase subunit alpha-1|C-P4Halpha(I)|collagen prolyl 4-hydroxylase alpha(I)|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I|procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1|prolyl 4-hydroxylase, alpha polypeptide I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P4HA1, check out the P4HA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P4HA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P4HA1 Antibody (A05917)
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No publications found for A05917
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