Product Info Summary
SKU: | M00300 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p62/SQSTM1 Rabbit Monoclonal Antibody
View all p62/SQSTM1 Antibodies
SKU/Catalog Number
M00300
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p62/SQSTM1 Rabbit Monoclonal Antibody catalog # M00300. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p62/SQSTM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00300)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EBB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p62/SQSTM1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00300 is reactive to SQSTM1 in Human, Mouse, Rat
Applications
M00300 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
62 kDa
Calculated molecular weight
47.687kDa
Background of p62/SQSTM1
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of p62/SQSTM1 using anti-p62/SQSTM1 antibody (M00300).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p62/SQSTM1 antigen affinity purified monoclonal antibody (Catalog # M00300) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p62/SQSTM1 at approximately 62 kDa. The expected band size for p62/SQSTM1 is at 48 kDa.
Click image to see more details
Figure 2. IHC analysis of p62/SQSTM1 using anti-p62/SQSTM1 antibody (M00300).
p62/SQSTM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-p62/SQSTM1 Antibody (M00300) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of p62/SQSTM1 using anti-p62/SQSTM1 antibody (M00300).
p62/SQSTM1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-p62/SQSTM1 Antibody (M00300) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis of Hela cells, using p62/SQSTM1 Antibody.
Click image to see more details
Figure 4. IHC analysis of p62/SQSTM1 using anti-p62/SQSTM1 antibody (M00300).
p62/SQSTM1 was detected in a paraffin-embedded section of human cervix squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-p62/SQSTM1 Antibody (M00300) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SQSTM1 (Source: Uniprot.org, NCBI)
Gene Name
SQSTM1
Full Name
Sequestosome-1
Weight
47.687kDa
Alternative Names
A170; Autophagy Receptor P62; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; ORCA; OSIL; oxidative stress induced like; p60PDB3; p62; p62B; Paget disease of bone 3; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome 1; sequestosome-1; SQSTM1; Ubiquitin-binding protein p62; ZIP3 SQSTM1 A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B sequestosome 1 sequestosome-1|EBI3-associated protein of 60 kDa|EBI3-associated protein p60|EBIAP|autophagy receptor p62|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa|ubiquitin-binding protein p62
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SQSTM1, check out the SQSTM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SQSTM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p62/SQSTM1 Rabbit Monoclonal Antibody (M00300)
Hello CJ!
M00300 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu X,Feng C,Wei G,Kong W,Meng H,Du Y,Li J.Mitofusin1 Is a Major Mediator in Glucose-Induced Epithelial-to-Mesenchymal Transition in Lung Adenocarcinoma Cells.Onco Targets Ther.2020 Apr 24;13:3511-3523.doi:10.2147/OTT.S238714.PMID:32425551;PMCID:PMC718794
Species: Human
M00300 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:500
Yuan H,Wang Y,Chen H,Cai X.Protective effect of flavonoids from Rosa roxburghii Tratt on myocardial cells via autophagy.3 Biotech.2020 Feb;10(2):58.doi:10.1007/s13205-019-2049-1.Epub 2020 Jan 22.PMID: 32015954;PMCID:PMC6976074.
Species: Rat
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
M00300 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:400
Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage
Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta
Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock?19/+ mice contributes to improved glucose homeostasis
Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-p62/SQSTM1 Rabbit Monoclonal Antibody
Question
My team were satisfied with the WB result of your anti-p62/SQSTM1 Rabbit Monoclonal antibody. However we have seen positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is SQSTM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
From what I have seen in literature, liver does express SQSTM1. Generally SQSTM1 expresses in cytoplasm, cytosol. Regarding which tissues have SQSTM1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8551575
Caudate nucleus, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 8650207, 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Pancreas, Placenta, Skin, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-11
Question
Is a blocking peptide available for product anti-p62/SQSTM1 Rabbit Monoclonal antibody (M00300)?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do provide the blocking peptide for product anti-p62/SQSTM1 Rabbit Monoclonal antibody (M00300). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
Will anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 work for IF with b-cell?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
According to the expression profile of b-cell, SQSTM1 is highly expressed in b-cell. So, it is likely that anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 will work for IF with b-cell.
Boster Scientific Support
Answered: 2019-09-30
Question
We have seen staining in mouse caudate nucleus trachea. What should we do? Is anti-p62/SQSTM1 Rabbit Monoclonal antibody supposed to stain caudate nucleus trachea positively?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
From what I have seen in literature caudate nucleus trachea does express SQSTM1. From what I have seen in Uniprot.org, SQSTM1 is expressed in left adrenal gland cortex, b-cell, cervix carcinoma, caudate nucleus trachea, pancreas, placenta, skin uterus, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SQSTM1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8551575
Caudate nucleus, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 8650207, 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Pancreas, Placenta, Skin, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-22
Question
Is this M00300 anti-p62/SQSTM1 Rabbit Monoclonal antibody reactive to the isotypes of SQSTM1?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of M00300 anti-p62/SQSTM1 Rabbit Monoclonal antibody is A synthesized peptide derived from human p62/SQSTM1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
I see that the anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 works with IF, what is the protocol used to produce the result images on the product page?
A. Jackson
Verified customer
Asked: 2018-09-19
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-19
Question
Is there a BSA free version of anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 available?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-02
Question
My question regarding product M00300, anti-p62/SQSTM1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Jones
Verified customer
Asked: 2017-09-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00300 anti-p62/SQSTM1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-06
Question
I was wanting to use to test anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 on mouse b-cell for research purposes, then I may be interested in using anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Walker
Verified customer
Asked: 2017-08-07
Answer
The products we sell, including anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-07
Question
Does M00300 anti-p62/SQSTM1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
As indicated on the product datasheet, M00300 anti-p62/SQSTM1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-03
Question
We purchased anti-p62/SQSTM1 Rabbit Monoclonal antibody for IF on placenta in the past. I am using mouse, and We are going to use the antibody for WB next. We need examining placenta as well as caudate nucleus trachea in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Anderson
Verified customer
Asked: 2016-05-20
Answer
I have checked the website and datasheets of our anti-p62/SQSTM1 Rabbit Monoclonal antibody and I see that M00300 has been validated on mouse in both IF and WB. Thus M00300 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-05-20
Question
We are currently using anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
R. Miller
Verified customer
Asked: 2016-04-20
Answer
The anti-p62/SQSTM1 Rabbit Monoclonal antibody (M00300) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-20
Question
I have attached the WB image, lot number and protocol we used for b-cell using anti-p62/SQSTM1 Rabbit Monoclonal antibody M00300. Please let me know if you require anything else.
Z. Mitchell
Verified customer
Asked: 2016-02-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-02
Question
I was wanting to use your anti-p62/SQSTM1 Rabbit Monoclonal antibody for IF for mouse b-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse b-cell identification?
D. Zhao
Verified customer
Asked: 2013-01-30
Answer
You can see on the product datasheet, M00300 anti-p62/SQSTM1 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse b-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-30