Product Info Summary
SKU: | A00454 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PAK1 (Ab-212) Antibody
SKU/Catalog Number
A00454
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PAK1 (Ab-212) Antibody (Catalog # A00454). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK1 (Ab-212) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00454)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Clone Number
8B1
Isotype
IgG
Immunogen
Peptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00454 is reactive to PAK1 in Human, Mouse, Rat
Applications
A00454 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
60.647kDa
Background of Pak1
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819
Thiel DA, et al. (2002) Curr Biol; 12:1227-1232
Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PAK1 using anti-PAK1 antibody (A00454).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (Catalog # A00454) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PAK1.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using PAK1(Ab-212) Antibody #A00454.
Click image to see more details
Figure 2. IHC analysis of PAK1 using anti-PAK1 antibody (A00454).
PAK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PAK1 Antibody (A00454) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PAK1 (Source: Uniprot.org, NCBI)
Gene Name
PAK1
Full Name
Serine/threonine-protein kinase PAK 1
Weight
60.647kDa
Superfamily
protein kinase superfamily
Alternative Names
alpha-PAK; EC 2.7.11; EC 2.7.11.1; MGC130000; MGC130001; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21-activated kinase 1; p65-PAK; PAK1; PAK-1; PAKalpha; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast PAK1 IDDMSSD, PAKalpha, alpha-PAK, p65-PAK p21 (RAC1) activated kinase 1 serine/threonine-protein kinase PAK 1|STE20 homolog, yeast|p21 protein (Cdc42/Rac)-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAK1, check out the PAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAK1 (Ab-212) Antibody (A00454)
Hello CJ!
No publications found for A00454
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-PAK1 (Ab-212) Antibody
Question
I see that the anti-PAK1 (Ab-212) antibody A00454 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-02
Question
My lab would like to test anti-PAK1 (Ab-212) antibody A00454 on mouse placenta for research purposes, then I may be interested in using anti-PAK1 (Ab-212) antibody A00454 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The products we sell, including anti-PAK1 (Ab-212) antibody A00454, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-13
Question
Our lab used your anti-PAK1 (Ab-212) antibody for IHC on brain in the past. I am using rat, and I plan to use the antibody for IF next. I was wanting to use examining brain as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
I viewed the website and datasheets of our anti-PAK1 (Ab-212) antibody and it appears that A00454 has been validated on rat in both IHC and IF. Thus A00454 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-19
Question
My team were content with the WB result of your anti-PAK1 (Ab-212) antibody. However we have observed positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is PAK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
From what I have seen in literature, liver does express PAK1. Generally PAK1 expresses in cytoplasm. Regarding which tissues have PAK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 23503467
Cervix carcinoma, Pubmed ID: 16964243, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 8805275
Boster Scientific Support
Answered: 2020-02-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-PAK1 (Ab-212) antibody A00454. Let me know if you need anything else.
C. Moore
Verified customer
Asked: 2020-01-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
I have a question about product A00454, anti-PAK1 (Ab-212) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00454 anti-PAK1 (Ab-212) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
Is this A00454 anti-PAK1 (Ab-212) antibody reactive to the isotypes of PAK1?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
The immunogen of A00454 anti-PAK1 (Ab-212) antibody is Peptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-19
Question
Does A00454 anti-PAK1 (Ab-212) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Mangal
Verified customer
Asked: 2019-05-17
Answer
As indicated on the product datasheet, A00454 anti-PAK1 (Ab-212) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-17
Question
I was wanting to use your anti-PAK1 (Ab-212) antibody for IHC for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
As indicated on the product datasheet, A00454 anti-PAK1 (Ab-212) antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-20
Question
We are currently using anti-PAK1 (Ab-212) antibody A00454 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
The anti-PAK1 (Ab-212) antibody (A00454) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-23
Question
We want using your anti-PAK1 (Ab-212) antibody for neuron projection morphogenesis studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
Thanks for your inquiry. This A00454 anti-PAK1 (Ab-212) antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-06
Question
Will anti-PAK1 (Ab-212) antibody A00454 work for IHC with placenta?
B. Banerjee
Verified customer
Asked: 2017-12-15
Answer
According to the expression profile of placenta, PAK1 is highly expressed in placenta. So, it is likely that anti-PAK1 (Ab-212) antibody A00454 will work for IHC with placenta.
Boster Scientific Support
Answered: 2017-12-15
Question
We have been able to see staining in rat leukemic t-cell. Do you have any suggestions? Is anti-PAK1 (Ab-212) antibody supposed to stain leukemic t-cell positively?
B. Gonzalez
Verified customer
Asked: 2017-01-09
Answer
Based on literature leukemic t-cell does express PAK1. Based on Uniprot.org, PAK1 is expressed in middle temporal gyrus, placenta, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, brain, among other tissues. Regarding which tissues have PAK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 23503467
Cervix carcinoma, Pubmed ID: 16964243, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 8805275
Boster Scientific Support
Answered: 2017-01-09
Question
Do you have a BSA free version of anti-PAK1 (Ab-212) antibody A00454 available?
W. Kulkarni
Verified customer
Asked: 2015-05-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAK1 (Ab-212) antibody A00454 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-13
Question
Please see the WB image, lot number and protocol we used for placenta using anti-PAK1 (Ab-212) antibody A00454. Please let me know if you require anything else.
R. Roberts
Verified customer
Asked: 2014-06-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-24
Question
Is a blocking peptide available for product anti-PAK1 (Ab-212) antibody (A00454)?
D. Li
Verified customer
Asked: 2013-05-20
Answer
We do provide the blocking peptide for product anti-PAK1 (Ab-212) antibody (A00454). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-20