Anti-Phospho-PAK1 (Thr84) Antibody

Product Name

Anti-Phospho-PAK1 (Thr84) Antibody

View all Pak1 Antibodies

SKU/Catalog Number

P00454-2

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-PAK1 (Thr84) Antibody (Catalog # P00454-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-PAK1 (Thr84) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00454-2)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 of human PAK1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00454-2 is reactive to PAK1 in Human, Mouse, Rat

Applications

P00454-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

60.647kDa

Background of Pak1

PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at Thr-84 and bind to gelsolin (Chen et al, 2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68 kDa PAK1 protein phosphorylated at Thr⁸⁴ in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase , 800 units/1mg protein for 30 minutes).

Gene/Protein Information For PAK1 (Source: Uniprot.org, NCBI)

Alternative Names

alpha-PAK; EC 2.7.11; EC 2.7.11.1; MGC130000; MGC130001; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21-activated kinase 1; p65-PAK; PAK1; PAK-1; PAKalpha; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast PAK1 IDDMSSD, PAKalpha, alpha-PAK, p65-PAK p21 (RAC1) activated kinase 1 serine/threonine-protein kinase PAK 1|STE20 homolog, yeast|p21 protein (Cdc42/Rac)-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)

For more info on PAK1, check out the PAK1 Infographic

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In this infographic, you will see the following information for PAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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