Anti-PAN2 Antibody Picoband™

Product Name

Anti-PAN2 Antibody Picoband™

View all USP52 Antibodies

SKU/Catalog Number

A03834-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAN2 Antibody Picoband™ catalog # A03834-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PAN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03834-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PAN2 recombinant protein (Position: M1-L356).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03834-1 is reactive to PAN2 in Human, Mouse, Rat

Applications

A03834-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

135 kDa

Calculated molecular weight

135.368kDa

Background of USP52

PAB-dependent poly(A)-specific ribonuclease subunit 2 is an enzyme that in humans is encoded by the PAN2 gene. This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PAN2 using anti-PAN2 antibody (A03834-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hel whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAN2 antigen affinity purified polyclonal antibody (Catalog # A03834-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAN2 at approximately 135 kDa. The expected band size for PAN2 is at 135 kDa.

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-PAN2 antibody (A03834-1).
Overlay histogram showing PC-3 cells stained with A03834-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAN2 Antibody (A03834-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PAN2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.13.4; FLJ39360; hPan2; Inactive ubiquitin carboxyl-terminal hydrolase 52; KIAA0710PAB-dependent poly(A)-specific ribonuclease subunit 2; NALP4; PABP-dependent poly(A) nuclease 2; PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae); PAN2 polyA specific ribonuclease subunit; PYPAF4; ubiquitin specific peptidase 52; ubiquitin specific protease 52; USP52 PAN2 USP52 poly(A) specific ribonuclease subunit PAN2 PAN2-PAN3 deadenylation complex catalytic subunit PAN2|PAB-dependent poly(A)-specific ribonuclease subunit 2|PAB-dependent poly(A)-specific ribonuclease subunit PAN2|PAB1P-dependent poly(A)-nuclease|PAB1P-dependent poly(A)-specific ribonuclease|PABP-dependent poly(A) nuclease 2|PAN deadenylation complex catalytic subunit 2|PAN deadenylation complex subunit 2|PAN2 poly(A) specific ribonuclease subunit homolog|inactive ubiquitin carboxyl-terminal hydrolase 52|poly(A)-nuclease deadenylation complex subunit 2|ubiquitin specific peptidase 52|ubiquitin specific protease 52

For more info on PAN2, check out the PAN2 Infographic

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In this infographic, you will see the following information for PAN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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