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Product Info Summary
| SKU: | M01588 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Parathyroid Hormone Receptor 1 Monoclonal Antibody
SKU/Catalog Number
M01588
BM5608 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Parathyroid Hormone Receptor 1 Monoclonal Antibody catalog # M01588. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parathyroid Hormone Receptor 1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01588)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEHO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Parathyroid Hormone Receptor 1 Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Reactive Species
M01588 is reactive to PTH1R in Human
Observed Molecular Weight
66 kDa
Calculated molecular weight
66.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01588 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: human SH-SY5Y whole cell, human A549 whole cell, human SIHA whole cell
Validation Images & Assay Conditions
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Western blot analysis of PTH1R using anti-PTH1R antibody (M01588).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH1R antigen affinity purified monoclonal antibody (M01588) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTH1R at approximately 66 kDa. The expected band size for PTH1R is at 66 kDa.
Specific Publications For Anti-Parathyroid Hormone Receptor 1 Monoclonal Antibody (M01588)
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