Anti-PARC CUL9 Antibody

Product Name

Anti-PARC CUL9 Antibody

View all CUL9 Antibodies

SKU/Catalog Number

A08481

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PARC CUL9 Antibody (Catalog # A08481). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-PARC CUL9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08481)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.

Reactive Species

A08481 is reactive to CUL9 in Human

Observed Molecular Weight

39 kDa

Calculated molecular weight

281.2 kDa

Background of CUL9

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08481 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000
Anti-PARC reacts with human and mouse PARC by western blot and immunoprecipitation.  The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others).  To date co-immunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected.  Most cell lines expressing PARC can be used as a positive control.  Researchers should determine optimal titers for other applications.

Validation Images & Assay Conditions

See full target information CUL9

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