Anti-PBK1 RSL1D1 Antibody

Product Name

Anti-PBK1 RSL1D1 Antibody

View all RSL1D1 Antibodies

SKU/Catalog Number

A07282

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PBK1 RSL1D1 Antibody (Catalog # A07282). Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-PBK1 RSL1D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07282)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A07282 is reactive to RSL1D1 in Human

Applications

A07282 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

54.973kDa

Background of RSL1D1

PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004). A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and activation of other protease systems involved in cell invasion (Tong et al., 2005). The cellular localization dynamics of PBK1, as well as the data from its yeast homologue (Cic1p/Nsa3p) suggest that PBK1 is essential for ribosome biogenesis (Fatica et al., 2003). Thus, PBK1 could be controlling cell proliferation by regulating the level of ribosome production. It is hypothesized that PBK1 is involved in regulating both, cell proliferation and invasiveness, therefore playing a dual function in cancer cells transformation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,500 - 1:10,000
IF Microscopy: 1:100
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1.In the overlay, specific antibody staining is shown to co-localize with recombinant protein.Cells were fixed with methanol prior to staining.Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

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Figure 2. Western blot analysis of RSL1D1 using anti-RSL1D1 antibody (A07282).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSL1D1 antigen affinity purified polyclonal antibody (Catalog # A07282) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RSL1D1.

Gene/Protein Information For RSL1D1 (Source: Uniprot.org, NCBI)

Alternative Names

CATX11; CATX-11; Cellular senescence-inhibited gene protein; CSIGMGC142259; DKFZP564M182; L12; MGC138433; PBK1cellular senescence inhibited gene protein; Protein PBK1; ribosomal L1 domain containing 1; ribosomal L1 domain-containing protein 1 RSL1D1 CSIG, L12, PBK1, UTP30 ribosomal L1 domain containing 1 ribosomal L1 domain-containing protein 1|CATX-11|cellular senescence-inhibited gene protein

For more info on RSL1D1, check out the RSL1D1 Infographic

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In this infographic, you will see the following information for RSL1D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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