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Product Info Summary
| SKU: | M01130 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PBRM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01130
BM5780 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PBRM1 Rabbit Monoclonal Antibody catalog # M01130. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PBRM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01130)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFHG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PBRM1
Reactive Species
M01130 is reactive to PBRM1 in Human, Mouse, Rat
Observed Molecular Weight
193 kDa
Calculated molecular weight
192.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01130 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HeLa cell
IHC: mouse thymus tissue, rat thymus tissue, human liver cancer tissu, human renal cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of PBRM1 expression in HeLa cell lysate.
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IHC analysis of PBRM1 using anti-PBRM1 antibody (M01130).
PBRM1 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of PBRM1 using anti-PBRM1 antibody (M01130).
PBRM1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of PBRM1 using anti-PBRM1 antibody (M01130).
PBRM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of PBRM1 using anti-PBRM1 antibody (M01130).
PBRM1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-PBRM1 Rabbit Monoclonal Antibody (M01130)
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Customer Q&As
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6 Customer Q&As for Anti-PBRM1 Rabbit Monoclonal Antibody
Question
Does anti-PBRM1 Monoclonal antibody M01130 work for Flow Cytometry with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PBRM1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-PBRM1 Monoclonal antibody M01130 will work for Flow Cytometry with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-02-17
Question
What sample was used in your WB analysis of M01130?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-PBRM1 Rabbit Monoclonal Antibody (M01130), western blot analysis of PBRM1 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
I am interested in to test anti-PBRM1 Monoclonal antibody M01130 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-PBRM1 Monoclonal antibody M01130 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-PBRM1 Monoclonal antibody M01130, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
Is there a BSA free version of anti-PBRM1 Monoclonal antibody M01130 available?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PBRM1 Monoclonal antibody M01130 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-23
Question
Is a blocking peptide available for product anti-PBRM1 Monoclonal antibody (M01130)?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
We do provide the blocking peptide for product anti-PBRM1 Monoclonal antibody (M01130). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-03-06
Question
We are currently using anti-PBRM1 Monoclonal antibody M01130 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
The anti-PBRM1 Monoclonal antibody (M01130) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-03
