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Product Info Summary
| SKU: | M04772 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PCK2 Monoclonal Antibody
SKU/Catalog Number
M04772
BM5430 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PCK2 Monoclonal Antibody catalog # M04772. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04772)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADFB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PCK2 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Reactive Species
M04772 is reactive to PCK2 in Human, Mouse, Rat
Observed Molecular Weight
71 kDa
Calculated molecular weight
70.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04772 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of PCK2 using anti-PCK2 antibody (M04772).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human LNCAP whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified monoclonal antibody (M04772) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71 kDa. The expected band size for PCK2 is at 71 kDa.
Specific Publications For Anti-PCK2 Monoclonal Antibody (M04772)
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7 Customer Q&As for Anti-PCK2 Monoclonal Antibody
Question
Can you help my question with product M04772, anti-PCK2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04772 anti-PCK2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
I was wanting to use your anti-PCK2 Monoclonal antibody for IF for mouse neuroblastoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse neuroblastoma identification?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
It shows on the product datasheet, M04772 anti-PCK2 Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse neuroblastoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-04
Question
Please see the WB image, lot number and protocol we used for neuroblastoma using anti-PCK2 Monoclonal antibody M04772. Please let me know if you require anything else.
J. Yang
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-PCK2 Monoclonal antibody M04772 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
N. Jones
Verified customer
Asked: 2018-10-23
Answer
The anti-PCK2 Monoclonal antibody (M04772) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-23
Question
Is there a BSA free version of anti-PCK2 Monoclonal antibody M04772 available?
B. Singh
Verified customer
Asked: 2018-07-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PCK2 Monoclonal antibody M04772 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neuroblastoma using anti-PCK2 Monoclonal antibody M04772. Let me know if you need anything else.
R. Lewis
Verified customer
Asked: 2016-05-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-05
Question
Is a blocking peptide available for product anti-PCK2 Monoclonal antibody (M04772)?
O. Zhao
Verified customer
Asked: 2014-12-19
Answer
We do provide the blocking peptide for product anti-PCK2 Monoclonal antibody (M04772). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-12-19
