Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody

IGF2BP3 expression was significantly associated with PD-L1 expression. A heatmap of immune checkpoints based on IGF2BP3 expression. B - F the correlation between IGF2BP3 expression and CD274 (PDL-1), PDCD1LG2 (PDL-2), LAG3, CTLA4, and HAVCR2. G, PD-L1 mRNA expression increased by IGF2BP3 upregulation
Index in PubMed under a CC BY license. PMID: 36732736

Western blot analysis of PD-L1 using anti-PD-L1 antibody (M00109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Ramos whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified monoclonal antibody (Catalog # M00109) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 40 kDa. The expected band size for PD-L1 is at 33 kDa.

Immunohistochemical analysis of paraffin-embedded human thymoma, using PD-L1 (CD274) Antibody.

Western blot analysis of PD-L1 using anti-PD-L1 antibody (M00109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human periodontal ligament fibroblasts whole cell lysates,
Lane 2: TGF-β1-induced with human periodontal ligament fibroblasts whole cell lysates,
Lane 3: Brefeldin A-treated with human periodontal ligament fibroblasts whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified monoclonal antibody (Catalog # M00109) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for PD-L1 at approximately 40-50 kDa. The expected band size for PD-L1 is at 33 kDa.