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- Table of Contents
CD274 antibody (M00109) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Immunohistochemical analysis of paraffin-embedded human thymoma, using PD-L1 (CD274) Antibody.
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Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody
CD274 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
CD274 antibody was positively detected by WB in:
human Ramos whole cell, human HepG2 whole cell, human THP-1 whole cell, human K562 whole cell, rat thymus tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD274 antibody was positively detected by IHC in:
human thymoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody
View all CD274 Antibodies
View all CD274 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IDC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PD-L1 (CD274)
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated Molecular Weight
33.3 kDa
Cite This Product
Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109)
Protocols
Western Blot protocol for CD274 antibody
Immunohistochemistry (IHC) protocol for CD274 antibody
Publications
Application:
Species:
Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis
Published: 2026-01-02
Journal: FITOTERAPIA
Application: WB,IHC
Impact Factor: 2.60
Species: Mouse
PMID: 41485690
Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma
Published: 2024-02-01
Journal: Journal for ImmunoTherapy of Cancer
Application: IHC
Impact Factor: 10.30
Species: Human
PMID: 38355278
IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer
Published: 2023-02-03
Journal: BMC CANCER
Application: IHC
Impact Factor: 3.80
Species: Human
PMID: 36732736
Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody
Published: 2020-09-18
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB,IHC
Impact Factor: 4.55
Species: Mouse,Human
PMID: 33152901
CD25 and TGF-β blockade based on predictive integrated immune ratio inhibits tumor growth in pancreatic cancer
Published: 2018-10-25
Journal: Journal of Translational Medicine
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 30359281
Expression of B7 Homolog 1 (B7H1) Is Associated with Clinicopathologic Features in Urothelial Bladder Cancer
Published: 2018-10-13
Journal: MEDICAL SCIENCE MONITOR
Application: IHC
Impact Factor: 1.89
Species: Human
PMID: 30315148
Effect of TLR4 and B7-H1 on immune escape of urothelial bladder cancer and its clinical significance.
Published: 2014-01-01
Journal: Asian Pacific Journal of Cancer Prevention
Application: IHC
Impact Factor: 1.50
Species: Human
PMID: 24606459
Review
Have you used Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody
The antibody shows a clear target band at the correct position, and the results are good.
| SKU | M00297 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampal tissue |
| Sample Processing Description | An Alzheimer’s disease (AD) model was induced by intracerebroventricular injection of streptozotocin (STZ) in mice. The mice were subsequently treated with four different doses of Qianlenta Heji, and total protein was extracted from the hippocampal tissues. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | c-Fos Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | c-FOS serves as a marker of neuronal activation under pathological conditions. Its expression is low in normal brain tissue but elevated in AD model mice, and is reduced after treatment. In the WB assay, lane 1 corresponds to hippocampal tissue from normal mice, lane 2 to AD model mice, lanes 3–5 to hippocampi treated with increasing doses of Qianlenta Heji, and lane 6 to a positive control drug. The results demonstrate that Qianlenta Heji exhibits therapeutic effects in the AD model. |
Xinru Qi, Shandong Second Medical University
Verified customer
Submitted 2025-12-11
Product Promise
We promise Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00109
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026