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- Table of Contents
PDCD1 antibody (M00178-4) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.
Red: PD1 Antibody [4D6] (M00178-4)
Blue: DAPI staining
Red: PD1 Antibody [4D6] (M00178-4)
Blue: DAPI staining
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Anti-PD1 PDCD1 Monoclonal Antibody [4D6]
PDCD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
PD-1 antibody can be used for detection of PD-1 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PD1 PDCD1 Monoclonal Antibody [4D6]
View all PDCD1 Antibodies
View all PDCD1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Mouse
Contents
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 4D6
Isotype
IgG1
Immunogen
PD-1 antibody was raised against the extracellular domain of human PD-1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
31.6 kDa
Background of PDCD1
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.
Cite This Product
Anti-PD1 PDCD1 Monoclonal Antibody [4D6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00178-4)
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Product Promise
We promise Anti-PD1 PDCD1 Monoclonal Antibody [4D6] performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00178-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026