This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
PDIA6 antibody (M03813-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Flow Cytometry analysis of SiHa cells using anti-PDIA6 antibody (M03813-1).
Overlay histogram showing SiHa cells stained with M03813-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PDIA6 Antibody (M03813-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with M03813-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PDIA6 Antibody (M03813-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7)
PDIA6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PDIA6 antibody was positively detected by WB in:
human K562 whole cell, human HepG2 whole cell, human HT1080, monkey COS-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PDIA6 antibody was positively detected by IHC in:
human bladder epithelial carcinoma tissue, human breast infiltrating ductal carcinoma tissue, human colorectal adenocarcinoma tissue, human endometrial cancer tissue, human laryngeal squamous cell carcinomas tissue, human liver cancer tissue, human ovarian cancer tissue, human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PDIA6 antibody was positively detected by ICC/IF in:
HepG2 cell
PDIA6 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Tested
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7)
View all PDIA6 Antibodies
View all PDIA6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3H5E7
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human PDIA6 recombinant protein (Position: L20-L440).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
48 kDa
Calculated Molecular Weight
48.1 kDa
Background of PDIA6
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03813-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Cow
97%
High Homology
Sheep
97%
High Homology
Mouse
96%
High Homology
Rat
95%
High Homology
Dog
95%
High Homology
Rabbit
93%
High Homology
Zebrafish
78%
Moderate Homology
Drosophila
61%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PDIA6 antibody
Immunohistochemistry (IHC) protocol for PDIA6 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PDIA6 antibody
Flow Cytometry (FC) protocol for PDIA6 antibody
Review
Have you used Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M03813-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026