Anti-PDK1 (Ab-241) PDPK1 Antibody

PDK-1 antibody

Boster Bio Anti-PDK1 (Ab-241) PDPK1 Antibody (Catalog # A01159). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01159
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-PDK1 (Ab-241) PDPK1 Antibody

View all PDK-1 Antibodies

SKU/Catalog Number

A01159

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PDK1 (Ab-241) PDPK1 Antibody (Catalog # A01159). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDK1 (Ab-241) PDPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01159)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

2D3

Isotype

IgG

Immunogen

Peptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01159 is reactive to PDPK1 in Human, Mouse, Rat

Applications

A01159 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

63.152kDa

Background of PDK-1

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development

Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A; 101(33): 12130-5

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For PDPK1 (Source: Uniprot.org, NCBI)

Gene Name

PDPK1

Full Name

3-phosphoinositide-dependent protein kinase 1

Weight

63.152kDa

Superfamily

protein kinase superfamily

Alternative Names

3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; PDK1; PDK-1; PDPK1; PDPK2; PDPK2P; PkB kinase like gene 1; PKB kinase PDPK1 PDK1, PDPK2, PDPK2P, PRO0461 3-phosphoinositide dependent protein kinase 1 3-phosphoinositide-dependent protein kinase 1|3-phosphoinositide-dependent protein kinase 2 pseudogene|PkB kinase like gene 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDPK1, check out the PDPK1 Infographic

PDPK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A01159

100ul

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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