Anti-Periostin/Postn Antibody Picoband™

Periostin/OSF-2 antibody

Boster Bio Anti-Periostin/Postn Antibody Picoband™ catalog # A01378. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A01378
Size: 100ug/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Periostin/Postn Antibody Picoband™

View all Periostin/OSF-2 Antibodies

SKU/Catalog Number

A01378

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-Periostin/Postn Antibody Picoband™ catalog # A01378. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Periostin/Postn Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01378)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived rat Periostin recombinant protein (Position: K577-Q810).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01378 is reactive to Postn in Mouse, Rat

Applications

A01378 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of Periostin/OSF-2

POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. It was found that purified recombinant PN supported adhesion of ovarian epithelial cells. And periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. It was showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Postn (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15063

Gene ID

10631

Gene Name

Postn

Full Name

Periostin

Weight

93.314kDa

Alternative Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 POSTN OSF-2, OSF2, PDLPOSTN, PN periostin periostin|osteoblast specific factor 2 (fasciclin I-like)|periodontal ligament-specific periostin|periostin, osteoblast specific factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Postn, check out the Postn Infographic

Postn infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Postn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A01378

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4 Customer Q&As for Anti-Periostin/Postn Antibody Picoband™

Question

We bought anti-Periostin/Postn antibody for ELISA on pubmed:8363580} in a previous experiment. I am using rat, and I plan to use the antibody for IHC next. My question regards examining pubmed:8363580} as well as pubmed:8363580} rc placenta eco:0000269 in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

I looked at the website and datasheets of our anti-Periostin/Postn antibody and it seems that A01378 has been tested on rat in both ELISA and IHC. Thus A01378 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-05-04

Question

Our lab were satisfied with the WB result of your anti-Periostin/Postn antibody. However we have observed positive staining in osteosarcoma {eco:0000269 golgi apparatus using this antibody. Is that expected? Could you tell me where is POSTN supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

According to literature, osteosarcoma eco:0000269 does express POSTN. Generally POSTN expresses in golgi apparatus. Regarding which tissues have POSTN expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Osteosarcoma, and Placenta, Pubmed ID: 8363580
Periodontal ligament, Pubmed ID: 15057823
Plasma, Pubmed ID: 16335952

Boster Scientific Support

Answered: 2019-09-24

Question

We are currently using anti-Periostin/Postn antibody A01378 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

The anti-Periostin/Postn antibody (A01378) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-12

Question

We have observed staining in rat liver. Any tips? Is anti-Periostin/Postn antibody supposed to stain liver positively?

C. Miller

Verified customer

Asked: 2016-11-30

Answer

Based on literature liver does express POSTN. Based on Uniprot.org, POSTN is expressed in periodontal ligament, osteosarcoma eco:0000269, pubmed:8363580 rc placenta eco:0000269, pubmed:8363580, plasma, liver, among other tissues. Regarding which tissues have POSTN expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Osteosarcoma, and Placenta, Pubmed ID: 8363580
Periodontal ligament, Pubmed ID: 15057823
Plasma, Pubmed ID: 16335952

Boster Scientific Support

Answered: 2016-11-30

Conjugation

how to order through PO

Size

Total: $315

Size:100ug

SKU:A01378

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A01378
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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